In vitro antioxidant activity of Morinda officinalis polysaccharides and its effect on solubility and stability of flavonoids
Objective:To explore the properties of Morinda officinalis polysaccharides and the effect on the solubility and stability of flavonoids in order to provide beneficial idea for the application of Morinda officinalis polysaccharides in the field of multi-component compatibility of traditional Chinese medicine.Methods:Firstly,the testing on the content,pH,protein content and molecular weight of Morinda officinalis polysaccharides was carried on.Secondly,the scavenging ability of Morinda officinalis polysaccharides on DPPH radicals and hydroxyl radicals was evaluated by in vitro experiments.Finally,the effects of Morinda officinalis polysaccharides on the solubility and stability of four common flavonoid monomer components with poor solubleness(quercetin,rutin,luteolin and luteoloside)in Eucommia ulmoides were investigated,and the interaction mechanism between them was investigated by using the phase solubility method.Results:Morinda officinalis polysaccharide is acidic,with a molecular weight of 1.97×103 Da.It has a certain ability to remove DPPH free radicals and hydroxyl radicals.It can form a 1:1 inclusion with 4 flavonoid components,which has a certain solubilization and stabilization effect.Under high temperature conditions,Morinda officinalis polysaccharide has a significant effect on promoting the stability of luteolin and luteoloside;under different pH conditions,it has a certain stabilizing effect on quercetin and luteolin,and it has a more significant stabilizing effect on luteoloside under alkaline conditions.Conclusion:This study provides a theoretical basis for the reasonable compatibility of Morinda officinalis and Eucommia ulmoides.
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