Protective effect of zingibroside R1 on LPS/D-GaIN-induced acute liver injury
Objective:Interventional effects of zingibroside R1 on acute liver injury and its mechanism of action.Methods:A liver injury mouse model was established by intraperitoneal injection of 10 pg/kg LPS and 700 mg/kg D-GalN,and the histopathological changes of the liver were observed by hematoxylin-eosin(HE).Biochemical methods were used to detect the levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),malondialdehyde(MDA)levels;small animal live imaging to observe the fluorescence intensity of 2-DG and PEG in mice;and flow cytometry to detect ROS and apoptosis levels in hepatocytes.Results:Compared with the model group,Zingibroside R1 improved liver pathological injury in mice with liver damage,significantly reduced the activity of serum ALT and AST,as well as the levels of 2-DG and PEG in the mice(P＜0.01),zingibroside R1 significantly reduced the levels of ROS in liver cells of mice with liver injury,as well as the contents of MDA in serum,and the levels of IL-1 β,TNF-α,IL-6,IFN-γ,and IL-2 in liver tissue(P＜0.01,P＜0.05).Zingibroside R1 also significantly increased the levels of SOD and GSH-Px(P＜0.05,P＜0.01).Conclusion:Zingibroside R1 exerts anti-hepatic injury effects through mediated oxidative stress and inflammation.
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