摘要
目的:姜状三七皂苷R1对急性肝损伤的干预作用及其作用机制.方法:通过腹腔注射10μg/kgLPS和700mg/kgD-GalN建立急性肝损伤小鼠模型,苏木素-伊红(HE)观察肝组织病理学变化;生化法检测谷草转氨酶(AST)、谷丙转氨酶(ALT)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、丙二醛(MDA)的水平;小动物活体成像观察2-DG和PEG在小鼠体内的荧光强度;流式细胞术检测肝细胞的活性氧和凋亡水平.结果:与模型组比较,各剂量姜状三七皂苷R1可改善急性肝损伤小鼠肝脏病理损伤;降低小鼠血清ALT、AST水平和小鼠体内2-DG和PEG的荧光强度(P＜0.01);降低肝细胞活性氧水平、血清MDA含量和小鼠肝脏组织中IL-iβ、TNF-a、IL-6、IFN-γ、IL-2的水平(P＜0.01,P＜0.05);提高SOD和GSH-Px水平(P＜0.05,P＜0.01).结论:姜状三七皂苷R1介导氧化应激和炎症来发挥抗急性肝损伤的作用.
Abstract
Objective:Interventional effects of zingibroside R1 on acute liver injury and its mechanism of action.Methods:A liver injury mouse model was established by intraperitoneal injection of 10 pg/kg LPS and 700 mg/kg D-GalN,and the histopathological changes of the liver were observed by hematoxylin-eosin(HE).Biochemical methods were used to detect the levels of aspartate aminotransferase(AST),alanine aminotransferase(ALT),glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),malondialdehyde(MDA)levels;small animal live imaging to observe the fluorescence intensity of 2-DG and PEG in mice;and flow cytometry to detect ROS and apoptosis levels in hepatocytes.Results:Compared with the model group,Zingibroside R1 improved liver pathological injury in mice with liver damage,significantly reduced the activity of serum ALT and AST,as well as the levels of 2-DG and PEG in the mice(P＜0.01),zingibroside R1 significantly reduced the levels of ROS in liver cells of mice with liver injury,as well as the contents of MDA in serum,and the levels of IL-1 β,TNF-α,IL-6,IFN-γ,and IL-2 in liver tissue(P＜0.01,P＜0.05).Zingibroside R1 also significantly increased the levels of SOD and GSH-Px(P＜0.05,P＜0.01).Conclusion:Zingibroside R1 exerts anti-hepatic injury effects through mediated oxidative stress and inflammation.
基金项目
国家重点研发计划中医药现代化研究专项(2019YFC1708800)
河南省科技重大专项(171100310500)
河南省高层次人才特殊支持计划"中原千人计划"中原领军人才项目(ZYQR201810080)
NETL
NSTL
万方数据