Effects of Bushen Tongshi Pills on osteogenic dysfunction of alcohol-induced femoral head necrosis rats by regulating ferroptosis mediated through activating Nrf2/SLC7A11/GPX4 pathway
Objective:To investigate the mechanism of Bushen Tongshi Pills in promoting the repair of alcoholic femoral head necrosis(AIONFH)in rats by inhibiting ferroptosis of osteoblasts.Methods:Thirty-six male SD rats were randomly divided into control group,model group and treatment group(Bushen Tongshi Pills,1.2g/kg),12 rats in each group were fed Lieber-DeCarli alcohol liquid feed to prepare alcoholic femoral head necrosis rat model.The relevant bone tissue scores were measured by Micro-CT scan.Gross morphology of femoral head was observed by naked eye.The histopathology of femoral head was observed by HE staining.The concentrations of malondialdehyde(MDA),glutathione(GSH)and total iron ions in serum and femoral head tissue were detected by biochemical kit.The mRNA and protein expressions of nuclear erythroid transcription factor 2(Nrf2),solute carrier family 7 member 11(SLC7A11),glutathione peroxidase 4(GPX4),alkaline phosphatase(ALP),osteocalcin(OCN)and Collagen-Ⅰ were detected by RT-qPCR,Western Blot analysis.Results:Compared with the model group,the bone loss and histopathological changes of the femoral head in the treatment group were alleviated(P＜0.05),the concentration levels of MDA and total iron ions in serum and femoral head tissue were significantly decreased(P＜0.05),and the level of GSH was significantly increased(P＜0.05).The mRNA and protein relative expressions of Nrf2,SLC7A11,GPX4,ALP,OCN and Collagen-Ⅰ were significantly increased(P＜0.05).Conclusion:Bushen Tongshi Pills may inhibit ferroptosis of osteoblasts by activating Nrf2/SLC7A11/GPX4 pathway,and promote osteogenic repair ability of femoral head in AIONFH rats.
Bushen Tongshi PillsAlcohol-induced osteonecrosis of the femoral head(AIONFH)OsteoblastFerroptosisNrf2/SLC7A11/GPX4 pathway
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