从中药材料学角度研究莲子心抗肿瘤药效物质基础
Research on material basis of lotus plumule for anti-tumor from medicine-hebology perspective
杨妮 1曹鹏 1陈景1
作者信息
摘要
目的:以中药材料学角度研究莲子心抗肿瘤药效物质基础.方法:通过动态光散射仪(DLS)和透射电镜(TEM)对甲基莲心碱/锰离子/人血清白蛋白复合纳米粒的粒径和形态进行表征.分别采用CCK-8法和活性氧检测试剂盒评估甲基莲心碱/锰离子/人血清白蛋白复合纳米粒对4T1细胞的毒性和产生活性氧的能力.分别将脂多糖、白介素-4、甲基莲心碱、锰离子和甲基莲心碱/锰离子/人血清白蛋白复合纳米粒与RAW264.7细胞共孵育24 h,并采用流式细胞术检测M1和M2型巨噬细胞占比来评估甲基莲心碱/锰离子/人血清白蛋白复合纳米粒重编程巨噬细胞的能力.进一步构建Balb/c小鼠4T1肿瘤模型,并对甲基莲心碱/锰离子/人血清白蛋白复合纳米粒的体内抗肿瘤活性进行评估.结果:甲基莲心碱/锰离子/人血清白蛋白复合纳米粒粒径约为148 nm呈圆形均匀分布.CCK-8实验结果显示:甲基莲心碱/锰离子/人血清白蛋白复合纳米粒对4T1细胞存在浓度依赖性细胞杀伤.进一步的机制研究结果表明:与甲基莲心碱组比较,其能提高4T1细胞内活性氧水平,高效抑制M2型巨噬细胞的极化.体内抗肿瘤实验证实了甲基莲心碱/锰离子/人血清白蛋白复合纳米粒抗肿瘤疗效强于甲基莲心碱(P＜0.05).结论:甲基莲心碱/锰离子/人血清白蛋白复合纳米粒被证实是莲子心抗肿瘤新型药效物质.
Abstract
Objective:To study the material basis of the anti-tumor efficacy of lotus plumule from the perspective of medicine-hebology.Methods:The particle size and morphology of neferine/manganese ion/human serum albumin composite nanoparticle was characterized by dynamic light scattering(DLS)and transmission electron microscope(TEM).The toxicity and ability to generate reactive oxygen species of neferine/manganese ion/human serum albumin composite nanoparticle on 4T1 cells was evaluated by CCK-8 assay and reactive oxygen species assay kit,respectively.The lipopolysaccharide,interleukin-4,neferine,manganese ion and neferine/manganese ion/human serum albumin composite nanoparticle were co-incubated with RAW264.7 cells for 24 h each and the ability of the neferine/manganese ion/human serum albumin composite nanoparticle to reprogramme macrophages was evaluated by detecting the percentage of M1 and M2 type macrophages using flow cytometry.4T1 tumor-bearing Balb/c mouse model was used to further evaluate the antitumor ability of neferine/manganese ion/human serum albumin composite nanoparticles in vivo.Results:In ultrapure water,the hydrodynamic diameter of neferine/manganese ion/human serum albumin composite nanoparticle was about 148 nm,The result of TEM showed that the neferine/manganese ion/human serum albumin composite nanoparticle was rounded and uniformly distributed.The CCK-8 experiments showed that there was a concentration-dependent cell killing of 4T1 cells by neferine/manganese ion/human serum albumin composite nanoparticle.Further mechanistic findings showed that it increased intracellular reactive oxygen species levels in 4T1 cells and effectively inhibited M2-type macrophage polarisation compared to the free neferine group.In vivo anti-tumor experiments confirmed that the anti-tumor efficacy of neferine/manganese ion/human serum albumin composite nanoparticles was stronger than that of neferine(P＜0.05).Conclusion:The neferine/manganese ion/human serum albumin composite nanoparticle is found to be the novel anti-tumour pharmacodynamic substances of lotus plumule.
关键词
莲子心/甲基莲心碱/药效物质基础/抗肿瘤/中药材料学Key words
Lotus plumule/Neferine/Material basis/Anti-tumor/Medicine-hebology引用本文复制引用
基金项目
国家自然科学基金(82305052)
中国科协青年人才托举工程项目(2022-QNRC1-04)
江苏省特聘教授基金(013074004038)
出版年
2024
NETL
NSTL
万方数据