酸枣DNA条形码筛选、种质资源鉴定与遗传多样性分析
Specific DNA barcodes,germplasm resources,and genetic diversity of Ziziphus jujuba var.spinosa
王琳 1赵红艳 2杜宪臣 3郭希蕾 1郭庆梅1
作者信息
- 1. 山东中医药大学,济南 250355
- 2. 济南紫金玫瑰股份有限公司,济南 250400
- 3. 山东济南平阴县自然资源局,济南 250400
- 折叠
摘要
目的:筛选特异性DNA条形码,并利用DNA条形码对酸枣不同种质进行种质资源鉴定及遗传多样性分析.方法:利用PCR扩增获得不同种质酸枣的ITS2、psbA-trnH、matK和rbcL核酸序列.用Mega和DNAman软件进行多重比对分析,筛选出的理想DNA条形码序列进行遗传距离分析,用Kimura 2-Parameter(K2P)模型分析遗传距离,用邻接法(NJ)构建系统发育树.结果:ITS2,psbA-trnH,matK和rbcL的扩增、测序成功率均为100％.所得序列与NCBI数据库上酸枣序列进行对比,鉴定率均＞99％.其中psbA-trnH序列平均GC含量最低为31.9％,22个酸枣栽培种质的psbA-trnH序列一致率最低,psbA-trnH序列可能提供更多的变异信息.基于psbA-trnH序列构建的NJ系统发育树将酸枣种质分为两大类,自展支持率为100％.结论:psbA-trnH序列在酸枣种质中遗传多样性更高,更适用于酸枣不同种质亲缘关系研究.
Abstract
Objective:The specific DNA barcodes were screened,and the DNA barcodes were used to identify the germplasm resources and analyzed the genetic diversity of Ziziphus jujuba var.spinosa.Methods:The nucleic acid sequences of ITS2,psbA-trnH,matK and rbcL were amplified by PCR.Mega and DNAman software were used for multiple comparison analysis,K2P model was used to analyze genetic distance,and adjacency method(NJ)was used to construct phylogenetic tree.Results:The amplification and sequencing success rates of ITS2,psbA-trnH,matK and rbcL were 100％.The obtained sequence was compared with the sequence on the NCBI database,and the identification rate was greater than 99％.The average GC content of psbA sequence is 31.9％,the psbA-tmH sequence of 22 varieties of Z jujuba var.spinosa has the lowest consistency,and the psbA-trnH sequence may provide more variation information.The psbA-trnH sequence of new varieties of Z.jujuba var.spinosa were different.The NJ phylogenetic tree constructed based on the psbA-trnH sequence divides jujube germplasm into two categories,with a self expanding support rate of 100％.Conclusion:The psbA-tmH sequence has higher genetic diversity in jujube germplasm,making it more suitable for studying the genetic relationships among different jujube germplasm.
关键词
酸枣/种质资源/DNA条形码/遗传多样性/psbA-trnH序列Key words
Ziziphus jujuba var.spinosa/germplasm resource/DNA barcode/Genetic diversity/psbA-trnH引用本文复制引用
基金项目
山东省科技型中小企业创新能力提升工程项目(2022TSGC2181)
出版年
2024
NETL
NSTL
万方数据