Specific DNA barcodes,germplasm resources,and genetic diversity of Ziziphus jujuba var.spinosa
Objective:The specific DNA barcodes were screened,and the DNA barcodes were used to identify the germplasm resources and analyzed the genetic diversity of Ziziphus jujuba var.spinosa.Methods:The nucleic acid sequences of ITS2,psbA-trnH,matK and rbcL were amplified by PCR.Mega and DNAman software were used for multiple comparison analysis,K2P model was used to analyze genetic distance,and adjacency method(NJ)was used to construct phylogenetic tree.Results:The amplification and sequencing success rates of ITS2,psbA-trnH,matK and rbcL were 100％.The obtained sequence was compared with the sequence on the NCBI database,and the identification rate was greater than 99％.The average GC content of psbA sequence is 31.9％,the psbA-tmH sequence of 22 varieties of Z jujuba var.spinosa has the lowest consistency,and the psbA-trnH sequence may provide more variation information.The psbA-trnH sequence of new varieties of Z.jujuba var.spinosa were different.The NJ phylogenetic tree constructed based on the psbA-trnH sequence divides jujube germplasm into two categories,with a self expanding support rate of 100％.Conclusion:The psbA-tmH sequence has higher genetic diversity in jujube germplasm,making it more suitable for studying the genetic relationships among different jujube germplasm.
NETL
NSTL
万方数据
