Study on the protective effect of Glycyrrhiza uralensis Fisch.based on the model organism zebrafish on specific heterogeneous liver injury caused by Polygonum multiflorum Thunb.
Objective:Exploring whether Glycyrrhiza uralensis Fisch.can protect against inflammatory heterogeneous liver injury caused by Polygonum multiflorum Thunb.,and elucidating the rationality and feasibility of the compatibility of the two from the perspective of safety pharmacology.Methods:A specific heterogeneous liver injury model of zebrafish was established using lipopolysaccharide(LPS)combined with Polygonum multiflorum extract(PME)or emodin(EMO),and different concentrations of glycyrrhiza uralensis extract(GUE)or Glycyrrhiza uralensis Fisch.components isoglycyrrhetinic acid(ISL)/glycyrrhetinic acid(GA)were administered.The liver phenotype,neutrophil and macrophage migration behavior of zebrafish were observed for liver histopathological analysis,Measure the activity of liver alanine aminotransferase(ALT)and aspartate aminotransferase(AST),and investigate the mRNA level changes of FXR and SHP genes.Explore the protective effect of Glycyrrhiza uralensis Fisch.on inflammatory specific liver injury caused by Polygonum multiflorum Thunb.from the perspectives of extract and single component compatibility.Results:Under inflammatory stress,GUE,ISL and GA significantly inhibited model drug-induced zebrafish liver degeneration,reduction of total liver area and brightness and elevation of ALT and AST viability,while attenuating inflammatory responses associated with idiosyncratic liver injury,and decreasing the number of immune cells around the neural thalamus.Different concentrations of Glycyrrhiza uralensis Fisch.inhibited the decrease in mRNA levels of FXR and SHP genes induced by Polygonum multiflorum Thunb.under inflammatory stress.Conclusion:GUE and its major components had significant protective effects against PME and its major components on zebrafish liver injury in the inflammatory stress state,and the mechanism of their protective effects might be related to the FXR-SHP axis.