Study on the effect and mechanism of Jianpi Zhidong Decoction on glucose metabolism chronic in restraint stress Tourette syndrome model
Objective:To investigate the effects and its mechanism of Jianpi Zhidong Decoction on glucose metabolism in restraint stress Tourette syndrome(TS)rat model.Methods:Male SD rats were randomly divided into a blank group(n=6)and a model group(n=18).The model group was given IDPN(250 mg·kg-1·d-1)intraperitoneal injection for 7 days and chronic restraint for 21 days.The blank group was given normal saline(250 mg·kg-1·d-1)for 7 days.The model group was divided into three groups according to stereotype score,which were model group,Tiapride group and Jianpi Zhidong Decoction group,and gavaged with corresponding drug for 28 days.The content of glucose(GLU),Pyruvate(Pyruvate)and lactic acid(LD)in serum was detected by colorimetric method.The histopathological morphology of hippocampus was observed by hematoxylin eosin(HE).The expression of glucose transporter 1(GLUT1)and glucose transporter 3(GLUT3)in hippocampus was detected by immunohistochemical method.Results:Compared with blank group,the levels of GLU,Pyruvate and LD in model group were significantly increased(P＜0.05),the cell arrangement of hippocampus was scattered and loose,the nucleus atrophy and necrosis were observed,and the expression of GLUT1 and GLUT3 protein was significantly increased(P＜0.05).Compared with model group,the levels of GLU,Pyruvate and LD decreased after Jianpi Zhidong Decoction intervention,the pathological injury of hippocampus was alleviated,and the expression of GLUT1 and GLUT3 protein was significantly decreased(P＜0.05).Conclusion:Jianpi Zhidong Decoction can protect brain tissue,affect glucose metabolism and the expression of glucose transporter in hippocampus,and may be related to the mechanism of glucose metabolism reprogramming.
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