Baicalin improves cell edema caused by OGD/R by regulating TRPV4/AQP4 on human brain astrocytes
Objective:To investigate the effects of Baicalin(BA)on human astrocyte SVGp12 with oxygen-glucose depriration/reperfusion(OGD/R)-induced injury,to regulate ion channel transient receptor potential vanilloid 4(TRPV4)and aquaporin 4(AQP4)from the perspective of inhibiting calcium influx and cell edema.Methods:The safe drug concentration of BA on SVGp12 cells and the OGD/R cell model were screened.OGD/R(4 h/2 h)method was used to simulate the environment of ischemia and hypoxia in the brain,and the groups were set into Control group,Model group,and BA group(5 μmol/L).At the same time,TRPV4 inhibitor HC067047 and agonist GSK1016790A were added.The cell viability was determined by absorbance,the permeability of lactate dehydrogenase(LDH)by ELISA,the expression of TRPV4 and AQP4 by Western Blot and the concentration of Ca2+by flow cytometry.Results:BA had no effect on the viability of SVGp12 cells in the concentration range of 12.5 μmol/L and lower.Under the condition of OGD/R(4 h/2 h),the OD value of SVGp12 cells decreased by about 50％(P＜0.01).After OGD/R induced injury,the cell viability was the highest when BA concentration was 5 μmol/L(P＜0.01).BA could improve the activity of SVGp12 cells induced by GSK1016790A(P＜0.05).BA decreased LDH permeability of SVGp12 cells(P＜0.05).BA could inhibit the expression of TRPV4 and AQP4(P＜0.05).BA could decrease the Ca2+concentration in SVGp12 cells(P＜0.01).Conclusion:BA has a protective effect on OGD/R-induced SVGp12 cells,possibly by inhibiting the up-regulation of TRPV4 channel protein and AQP4 channel protein after OGD/R,thereby reducing Ca2+influx and inhibiting cell swelling.
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