Study on anti-RSV mechanism of Qingfei Jiedu Granules in regulating type Ⅰinterferon pathway
Objective:To explore the effect mechanism of Qingfei Jiedu Granules(QJG)on respiratory syncytial virus(RSV)based on type Ⅰ interferon pathway.Methods:Forty female BALB/c mice were randomly divided into control group(Con),model group(RSV),RSV+Qingfei Jiedu Granules group(RSV+QJG),RSV+IFN-α blocker group(RSV+IFN-α Inh).RSV+IFN-α blocker+Qingfei Jiedu Granules(RSV+IFN-α Inh+QJG).Except for Con group,other groups were given RSV strain,and RSV+IFN-α Inh group and RSV+IFN-α Inh+QJG group were given IFN-α blocker before two nasal drops.After infection,the treatment group was treated with QJG.The general state of mice in each group was observed,and the mice were sacrificed for sampling after 3 days of treatment.The pathological changes of lung tissues were observed by HE staining and scored.The serum levels of IFN-α/β were detected by ELISA.The transcription levels of RIG-I,MAVS,IFN-α/β,RSVF,RSVG and other genes in lung tissues of mice were detected by Q-PCR.The protein expression levels of RSV-F,IRF3,OAS1 and MX1 genes were detected by immunofluorescence and Western Blot.Results:Compared with RSV group,QJG drug therapy could significantly inhibit the level of RSV in lung tissue and improve the inflammatory injury of lung tissue(P＜0.0l).Compared with RSV group,the expression levels of RIG-I,MAVS,p-IRF3,IFN-α/β,OAS1 and MX1 genes in lung tissue of mice in RSV+QJG group were significantly increased(P＜0.01,P＜0.05).Intervention with IFN-α blockers could affect the antiviral effect of QJG and the elevated levels of IFN-α,OAS1,MX1 genes(P＜0.01,P＜0.05).Conclusion:QJG may exert anti-RSV effect by regulating type Ⅰ interferon pathway.
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