摘要
目的:运用血清代谢组学的方法探究益艾康胶囊治疗艾滋病免疫重建不良的作用机制.方法:纳入83例艾滋病免疫重建不良患者,其中肺脾气虚证患者58例,非肺脾气虚证患者25例.给予肺脾气虚证患者益艾康胶囊干预24周,用流式细胞技术检测各样本T淋巴细胞亚群及CD4+/CD8+细胞比例;利用质谱技术检测干预前后各组血清,对代谢组学数据分析整理.结果:益艾康胶囊干预后CD4+T细胞计数、CD4+/CD8+比值均显著升高(P＜0.05).肺脾气虚组和非肺脾气虚证组鉴别出代谢差异物296种,肺脾气虚证治疗前后鉴别出差异代谢物248种.将差异代谢产物进行代谢通路的富集分析,肺脾气虚证组和非肺脾气虚组、益艾康胶囊治疗前后共同差异代谢通路为硒氨基酸代谢、泛酸和CoA生物合成、磷酸肌醇代谢、类固醇激素的合成、烟酸酯和烟酰胺代谢、嘌呤代谢.共同的代谢通路命中的代谢物1-磷脂酰-D-巯基肌醇、PC[18∶3(9Z,12Z,15Z)/20∶1(11Z)]、PE(14∶0/P-18∶0)、喹啉酸、去氧皮质酮、硒代半胱氨酸.结论:益艾康胶囊可以促进免疫功能重建,1-磷脂酰-D-巯基肌醇、PC[18∶3(9Z,12Z,15Z)/20∶1(11Z)]、PE(14∶0/P-18∶0)、喹啉酸、去氧皮质酮、硒代半胱氨酸等代谢产物与益艾康胶囊治疗肺脾气虚证作用紧密相关,其所在代谢通路可能是益艾康胶囊治疗免疫重建不良肺脾气虚证潜在切入点.
Abstract
Objective:To preliminarily explore the mechanism of Yiaikang Capsules in the treatment of AIDS immune deficiency syndrome by UHPLC metabolomics technology.Methods:A total of 83 PIR patients were recruited in the study,including 25 patients without lung-spleen qi deficiency syndrome and 58 patients with lung-spleen qi deficiency syndrome.Intervention with Yiaikang Capsules for 24 weeks in patients with poor reconstruction of lung qi deficiency syndrome,Using UHPLC metabolomics technology to detect the different of each group,analyzing metabolomics data,screening differential metabolites,and useing enrichment and topological analysis to search for differential metabolic pathways.Results:CD4+T cell count,the expression rate of CD4+/CD8+were significantly increased(P＜0.05).A total of 296 differential metabolites were finally identified in the lung-spleen qi deficiency syndrome group and the without lung-spleen qi deficiency syndrome group;248 differential metabolites were finally identified after intervention.Enrichment analysis of metabolic pathways was conducted on differential metabolites,the common differential metabolic pathways between the lung-spleen qi deficiency group-the non-lung-spleen qi deficiency group,and before and after treatment with Yiaikang Capsules,were selenium amino acid metabolism,pantothenic acid and CoA biosynthesis,phosphoinositide metabolism,steroid hormone synthesis,nicotinic acid ester and nicotinamide metabolism,and purine metabolism.The metabolites hit by the common metabolic pathway are 1-phosphatidyl-D-methioinositol,PC[18∶3(9Z,12Z,15Z)/20∶1(11Z)],PE(14∶0/P-18∶0),quinolinic acid,deoxycorticosterone,and selenocysteine.Conclusion:Yiaikang Capsules can promote immune function reconstruction.1-phosphatidyl-D-methioinositol,PC[18∶3(9Z,12Z,15Z)/20∶1(11Z)],PE[14∶0/P-18∶0],quinolinic acid,deoxycorticosterone,selenocysteine,etc.are closely related to the therapeutic effect of Yiaikang Capsules on lung spleen qi deficiency syndrome.The metabolic pathway may be a potential entry point for the treatment of immune reconstitution dysfunction in lung spleen qi deficiency syndrome.
基金项目
国家自然科学基金(82274474)
河南省科技攻关计划(202102310165)
河南省科技攻关计划(222102310570)
河南省科技攻关计划(232102311207)
河南省中医药青苗人才培养项目(第二批)(豫卫中医函[2021]16号)
河南省中医学"双一流"创建科学研究专项(HSRP-DFCTCM-2023-3-22)
NETL
NSTL
万方数据