姜汁水凝胶穴位贴敷对哮喘大鼠辅助性T细胞2细胞因子的调节作用
Modulation of helper T cell 2 cytokines in asthmatic rats by acupoint application with ginger juice hydrogel
杨秋红 1卫弯 2田健材 2高慧 2董国启 2杨华元2
作者信息
- 1. 上海中医药大学,上海 201203;上海市虹口区江湾医院,上海 200080
- 2. 上海中医药大学,上海 201203
- 折叠
摘要
目的:制备Fe3O4/N-异丙基丙烯酰胺(PNIPAM)新型穴位敷贴剂型,检测其性能,并应用于哮喘模型的穴位贴敷疗法,探讨该疗法对哮喘大鼠辅助性T细胞2细胞因子调节作用.方法:制备Fe3O4/PNIPAM复合水凝胶并测定升温性能,电镜下观察内部结构,检验细胞毒性.将32只SD大鼠随机分为4组,分别为正常组、模型组、激光姜汁组、激素组,每组8只.除正常组外,其余组制备哮喘模型,激光姜汁组姜汁水凝胶贴敷大椎穴5 min并808 nm激光照射控制药物释放;激素组采用地塞米松1.2 mg/kg腹腔注射;均为隔日1次.观察大鼠一般状态;ELESA检测支气管肺泡灌洗液(BALF)中免疫球蛋白E(IgE)、白介素(IL)-4、IL-13的表达;HE染色检测肺组织病理情况.结果:2.0 Fe3O4/PNIPAM具有疏松孔状结构,良好的吸热升温性能,且无细胞毒性.与模型组比较,激光姜汁组可显著改善哮喘大鼠的行为学评分(P＜0.01);与模型组和激素组比较,激光姜汁组显著降低BALF中IgE的含量,降低IL-4、IL-13的表达(P＜0.01,P＜0.05).结论:Fe3O4/PNIPAM无细胞毒性,具有良好的疏松孔状结构可负载药物,具有良好的吸热升温性能可控制药物释放,可作为新型穴位贴敷制剂.姜汁水凝胶穴位贴敷疗法可通过降低BALF中IgE、IL-4、IL-13的表达缓解哮喘的发作症状.
Abstract
Objective:To prepare a new type of acupoint application form of Fe3O4/PNIPAM,test its performance,and apply it to acupoint application therapy in asthma animal model,and explore the effect of this therapy on the regulation of helper T cell 2 cytokines in asthmatic rats.Methods:Fe3O4/PNIPAM composite hydrogel was prepared and the warming performance was determined,and the internal structure was observed under the electron microscope to test the cytotoxicity.Thirty-two SD rats were randomly divided into 4 groups,which were the normal group,the model group,the laser-ginger juice group,and the hormone group,8 in each group.Except for the normal group,asthma models were prepared for the other groups.In the laser-ginger juice group,ginger juice hydrogel was applied to the Dazhui(DU 14)for 5 min and 808 nm laser irradiation was used to control drug release;in the hormone group,dexamethasone was injected intraperitoneally at 1.2 mg/kg,and was administered once every other day.The general state of the rats was observed;the expression of IgE,IL-4 and IL-13 in BALF was detected by ELESA;and the pathology of lung tissue was detected by HE staining.Results:2.0 Fe3O4/PNIPAM had a loose pore-like structure,good heat-absorbing and warming properties,and no cytotoxicity.Compared with the model group,the laser-ginger juice group significantly improved the behavioral scores(P＜0.01).Compared with the model group or the hormone group,the laser-ginger juice group significantly reduced the content of IgE,and the expression of IL-4 and IL-13 in the BALF of the asthmatic rats(P＜0.0l,P＜0.05).Conclusion:Fe3O4/PNIPAM is non-cytotoxic,has a good loose pore structure for drug loading,and has good heat-absorbing and warming properties for controlling drug release,and can be used as a new type of acupoint application preparation.Ginger juice hydrogel acupoint application therapy can alleviate asthma attack symptoms by reducing the expression of IgE,IL-4,and IL-13 in BALF.
关键词
Fe3O4/N-异丙基丙烯酰胺/水凝胶/哮喘/穴位贴敷/制剂/姜汁Key words
Fe3O4/NIPAM/Hydrogel/Asthma/Acupoint application/Preparation/Ginger juice引用本文复制引用
基金项目
国家重点研发计划(2018YFC1707801)
上海市进一步加快中医药传承创新发展三年行动计划(2021-2023)(ZY2021-2023-0502)
上海市进一步加快中医药传承创新发展三年行动计划(2021-2023)(ZY2021-2023-0202)
出版年
2024
国家科技期刊平台
NSTL
万方数据