豨莶草水洗脱部位对H3N2型甲流病毒感染小鼠的作用及机制
Effects and mechanism of siegesbeckia water elution site on mice infected with H3N2 influenza A virus
王欢 1樊凯芳 1陈媛 1王腾岳 1赵铭岳1
作者信息
摘要
目的:探讨豨莶草水洗脱部位(SWES)对BALB/c小鼠H3N2型甲流病毒所致呼吸道感染的作用及机制.方法:以50只BALB/c小鼠为研究对象,其中42只采用限食法(100g饲料/kg-1·d-1)进行小鼠气虚模型建立;另取8只小鼠,正常饲喂,作为正常对照组.选择模型显著40只随机分为模型对照组,奥司他韦胶囊组,连花清瘟胶囊组,SWES低、高剂量组,每组8只.各组小鼠连续灌胃相应药物7 d,除正常对照组外,各组小鼠于给药第4天给药前2 h经鼻滴入H3N2型甲型流感病毒原液(60 μL/只)进行感染,感染后72 h,检测各组小鼠肺脏、胸腺、脾指数,光镜观察各组小鼠肺组织病理变化,采用ELISA法检测各组小鼠肺脏中白介素(IL)-6、IL-1β、肿瘤坏死因子(TNF-α)的含量,采用半定量PCR检测各组小鼠肺组织病毒载量;采用免疫组化法检测肺脏组织α7nAchR表达量;采用Western blot法检测肺脏组织P65、p-P65蛋白表达量.结果:与模型对照组比较,SWES高剂量组能显著减轻小鼠感染甲流病毒后的肺组织病理学改变,降低肺组织评分(P＜0.05),显著降低感染小鼠肺组织病毒载量(P＜0.01),以及小鼠肺脏中IL-6、IL-1β、TNF-α的含量(P＜0.01);SWES低、高剂量组能显著升高甲流病毒感染肺α7nAchR的表达(P＜0.05),显著降低甲流病毒感染肺组织p-P65蛋白的表达(P＜0.05).结论:SWES具有抗甲流病毒的药效作用,其抗甲流病毒感染的作用机制可能与其调节肺组织a7nAchR/P65信号通路,减少肺组织炎症因子(IL-6、IL-1β)的分泌有关.
Abstract
Objective:To explore the effects and mechanism of siegesbeckia water elution site(SWES)on respiratory tract infection caused by influenza A virus in BALB/c mice.Methods:Fifty BALB/c mice were taken as the research object,42 of them were used to establish the model of qi deficiency by food restriction(100 g feed·kg-1·d-1),and 8 of them were taken as the normal control group.Forty models were selected and randomly divided into model group,Oseltamivir Capsule group,Lianhua Qingwen Capsule group,low dose SWES group and high dose SWES group,8 in each group.The mice in each group were administered by gavage for 7 consecutive days.Except the normal control group,the mice in each group were intranasally drip H3N2 type A influenza virus stock solution before 2 hours administration on the fourth day of administration 60 μL/infection only,72 h after infection,the indexes of lung,thymus and spleen of mice in each group were detected,the pathological changes of lung tissue in mice in each group were observed under light microscope,and IL-6 and IL-1 β,TNF-α in lungs of mice in each group were detected by ELISA,the viral load in lung tissue of mice in each group was detected by Semi quantitative PCR;Detection α 7nAchR expression of lung tissue was detected by immunohistochemistry;the expression of P65 and p-P65 protein in lung tissue were detected by Western blot.Results:Compared with the model group,SWES high-dose group can significantly reduce the pathological changes of lung tissue after infected with influenza A virus,and significantly reduce the viral load in lung tissue of infected mice(P＜0.05),and significantly reduce contents of IL-6 and IL-1 β,TNF-α in lung of mice(P＜0.01);SWES low-,high-dose group dose group can significantly increase the expression of α 7nAchR of the pulmonary infection of influenza A virus(P＜0.05),significantly decreased the expression of p-P65 protein in lung tissues infected with HAV(P＜0.05).Conclusion:SWES has the effects of anti influenza virus,and its mechanism of anti influenza virus infection may be related to its regulation of a7nAchR-P65 signal pathway in lung tissue and reduction of the secretion of inflammatory factors(IL-6,IL-1 β)in lung tissue.
关键词
豨莶草/水洗脱部位/H3N2型甲流病毒/呼吸道感染Key words
Siegesbeckia/Water elution site/H3N2 influenza A virus/Respiratory tract infection引用本文复制引用
基金项目
山西省中医药局中医药创新团队项目(zyytd2024023)
山西省基础研究计划(20210302123230)
山西省中医药局科研项目(2023ZYYB043)
出版年
2024
国家科技期刊平台
NSTL
万方数据