Objective:To explore the pathogenesis of recurrent spontaneous abortion based on 4D label free quantitative proteomics technology.Methods:Eight patients with early recurrent spontaneous abortion and eight women who underwent non medical induced abortion were included.Protein extraction was performed on the decidual tissue of the subjects,followed by trypsin hydrolysis and liquid chromatography-mass spectrometry analysis to obtain raw data.Differential expression proteins were obtained based on screening criteria,and intersection mapping was performed for bioinformatics analysis.Results:A total of 5 063 proteins were identified in both groups,and a total of 580 differentially expressed proteins were screened,among which 244 proteins were up-regulated and 336 proteins were down-regulated.GO enrichment analysis showed that differential proteins were mainly concentrated in biological processes such as response to external stimulus,defense response,biological adhesion,cell motility,localization of cell,cell migration,multicellular organismal process,cell communication.The results of KEGG pathway enrichment analysis showed that differential proteins participated in PI3K-AKT,p53 signaling pathway and PPAR signaling pathway.Five core proteins,including FN1,FGF2,MPO,BGN and SPP1,were screened out in the protein interaction network.Conclusion:The mechanism of recurrent spontaneous abortion may involve biological processes and signaling pathways such as inflammation,oxidative stress,immunomodulation,coagulation regulation,and cell adhesion.The differential proteins and signaling pathways obtained by using 4D label-free quantitative proteomics technology and further bioinformatics analysis are of great significance for revealing the pathogenesis of recurrent miscarriage.
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