首页|基于PP2A/AKT通路探讨扶正祛毒汤对急性髓系白血病-完全缓解患者CD34+细胞源DC激发T细胞的影响

基于PP2A/AKT通路探讨扶正祛毒汤对急性髓系白血病-完全缓解患者CD34+细胞源DC激发T细胞的影响

Exploration on the effects of Fuzheng Qudu Decoction interventing AML-CR patients'CD34+cells source DC activate T cells based on PP2A/AKT signaling pathway

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目的:探讨扶正祛毒汤治疗急性髓系白血病-完全缓解(AML-CR)的可能作用机制.方法:运用血清药理学、Ficoll密度梯度离心、免疫磁珠阳性选择、细胞培养、流式细胞分析、Western Blot和RT-qPCR等技术检测DC表面CD1a、CD80、CD83、CD86、HLA-DR的表达,DC激发T细胞中CTLA-4、PP2A、AKT、p-AKT蛋白和mRNA的表达.结果:扶正祛毒汤含药血清能诱导AML-CR患者CD34+细胞形成典型的DC形态,显著增加DC表面CD1a、CD80、CD83、CD86、HLA-DR的表达(P＜0.05),上调DC激发T细胞中PP2A蛋白及mRNA的表达(P＜0.05),下调CTLA-4、AKT蛋白及mRNA的表达(P＜0.05),下调p-AKT蛋白表达(P＜0.05).结论:扶正祛毒汤治疗AML-CR的作用机制可能与该方能够促使AML-CR患者CD34+细胞源DC成熟,抑制CTLA-4与CD80、CD86的结合,有效激活T细胞,调控PP2A/AKT信号通路,进而杀伤白血病细胞有关.
Objective:To explore the possible mechanism of Fuzheng Qudu Decoction in the treatment of acute myeloid leukemi-complete remission(AML-CR).Methods:Serum pharmacology,Ficoll density gradient centrifugation,positive selection with immunomagnetic beads,cell culture,flow cytometric analysis,Western Blot and RT-qPCR were used to determine the expression of CDla,CD80,CD83,CD86 and HLA-DR on the surface of DC;and determine the expression of CTLA-4,PP2A,AKT,p-AKT protein and mRNA in DC activating T cells.Results:Serum containing drugs from Fuzheng Qudu Decoction induced the formation of typical DC morphology in CD34+cells from AML-CR patients,significantly increased the expression of CDla,CD80,CD83,CD86 and HLA-DR on the surface of DC(P＜0.05);upregulated the expression of PP2A protein and mRNA(P＜0.05),downregulated the expression of CTLA-4,AKT protein and mRNA(P＜0.05),and downregulated the expression of p-AKT protein in DC activating T cells(P＜0.05).Conclusion:The mechanism of Fuzheng Qudu Decoction in treating AML-CR may be related to its ability to promote the maturation of CD34+cell-derived DC in AML-CR patients,inhibit the combination of CTLA-4 with CD80 and CD86,effectively activate T cells,regulate PP2A/AKT signaling pathway,and then kill leukemia cells.

Acute myeloid leukemi-complete remission(AML-CR)Fuzheng Qudu DecoctionPP2A/AKT signaling pathwayDendritic cellsT cells

彭名行、黄礼明、周伟、朱国庆、尹尚瑾、左乐、陈孟豪

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贵州中医药大学,贵阳 550025

贵州中医药大学第二附属医院,贵阳 550003

急性髓系白血病-完全缓解 扶正祛毒汤 PP2A/AKT信号通路 树突细胞 T细胞

国家自然科学基金地区科学基金贵州省中医药局项目贵州中医药大学研究生教育创新计划(2022)贵州中医药大学研究生教育创新计划(2022)

81860801QZYY-2018-038YCXZRB202210YCXZRB202206

2024

中华中医药杂志
中华中医药学会

中华中医药杂志

CSTPCD北大核心
影响因子：1.135
ISSN：1673-1727
年,卷(期)：2024.39(6)
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