Effects of different meridian aromatic drugs on airway mucosa of influenza mice and analysis of differential gene expression profiling
Objective:To study the differences in airway mucosa and gene expression profiles of influenza mice by aromatic drugs with lung meridian or not.Methods:Forty BALB/c mice were randomly divided into blank group,model group,Patchouli group and Atractylodes group,10 mice in each group.Except for the blank group,FM1 virus was administered intranasally to establish an influenza model.The treatment group mice were nebulized with Patchouli or Atractylodes,and euthanized after 5 days of intervention.Samples were collected and tested.HE staining was used to observe the pathological morphology of lung and tracheal tissues,ELISA method was for detecting sIgA,TNF-α,IFN-γ and IL-1β in mouse alveolar lavage fluid,flow cytometry method was for detecting T lymphocyte,transcriptome sequencing analysis was for detecting differentially expressed genes in mouse lung tissue,and visualization display by using bioinformatics technology.Results:Both Patchouli and Atractylodes could alleviate inflammatory pathological damage to lung tissues in influenza mice.Compared with the model group,both Patchouli and Atractylodes group increased sIgA(P＜0.05),decreased TNF-α,IFN-γ and IL-1βexpression(except IL-1β expression in Atractylodes group)(P＜0.01,P＜0.05),elevated CD4+T,CD4+/CD8+and decreased CD8+T lymphocytes(P＜0.01,P＜0.05).Compared with Atractylodes group,the decrease of TNF-α,IFN-γ were more pronounced in Patchouli group(P＜0.01),the CD4+,CD8+T lymphocytes in the tracheal mucosa were significantly increased(P＜0.01).There were 1 103,311,and 315 differential genes in the model group,Patchouli group,and Atractylodes groups,respectively.The common pathways were different between in the three groups.For the IL-10 signaling pathway,the Patchouli group showed an activation effect,while the model group showed an inhibitory effect,MHC,CREB and BMP2 were the common molecules in the Patchouli group and model group.In the macrophage alternative activation signaling pathway,the Atractylodes group showed an inhibitory effect,while the model group showed an activation effect,IGG and CREB were the common molecules in the Atractylodes group and the model group.Conclusion:For the influenza,the effect of lung meridian aromatic drugs Patchouli may be more related to in the process of immune regulation and inflammation than the other meridian drugs Atractylodes,these innovative findings give a reference for the Chinese herbal meridian theory.
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万方数据
