Identification of Tibetan medicine Suoluogabu varieties based on DNA molecular identification and chemical pattern recognition methods
Objective:To identify of Tibetan medicine Suoluogabu based on DNA molecular identification and HPLC fingerprinting combined with chemical pattern recognition methods.Methods:The DNA of 20 batches of Suoluogabu medicinal herbs was extracted,different primer sequences by PCR and sequenced was amplified,and the appropriate primer sequences was screened,the sequence characteristics of medicinal materials was analyzed and a phylogenetic tree was constructed.HPLC was used to establish the fingerprints of Suoluogabu,and similarity evaluation,cluster analysis(CA),principal pattern analysis(PCA),and orthogonal partial least squares-discriminant analysis(OPLS-DA)were applied to investigate the chemical pattern recognition.Results:The ITS-18F/ITS-25R sequence and HPLC fingerprint could effectively divide 20 batches Suoluogabu into two categories:gonococcus and conglomerate.Conclusion:The method established by this institute can provide a reference for the identification,quality improvement,and application of Tibetan medicine Suoluogabu.
Tibetan medicineSuoluogabuDNA molecular identificationHPLC fingerprintChemical pattern recognitionVarieties identificationPhylogenetic tree
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NSTL
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