Preliminary study on the effects of serum exosome on the survival time of hypoxic mice after electroacupuncture in normal mice and its action law
Objective:To investigate the effects of serum exosome on the survival time of hypoxic mice after acupuncture in normal mice.Methods:KM mice were randomly divided into blank control group and electroacupuncture group.The hypoxia model was replicated immediately after electroacupuncture pretreatment,and the survival time was observed.KM mice were randomly divided into E0,E1,E4 and E7 groups,and acupuncture-exosome(Acu-exo)was extracted by ExoQuick method for identification.Acu-exo was infused back to reinfusion E0 group,reinfusion E1 group,reinfusion E4 group and reinfusion E7 group,respectively.After 30 min,the hypoxia model was replicated,and the survival time was observed.KM mice were randomly divided into blank control group,electroacupuncture group,electroacupuncture+DMSO group and electroacupuncture+GW4869 group.The mice in electroacupuncture+DMSO group and electroacupuncture+GW4869 group were given intraperitoneal injection of DMSO and GW4869,respectively.After 1 hour,the mice were pretreated with electroacupuncture and the hypoxia model was replicated immediately.The survival time was observed.Results:The survival time of electroacupuncture group was significantly longer than that of blank control group(P＜0.01).Compared with the blank control group,the hypoxia tolerance time of reinfusion E1 group,reinfusion E4 group and reinfusion E7 group was significantly improved(P＜0.01).Compared with the blank control group and electroacupuncture+GW4869 group,the survival time of the electroacupuncture group was significantly improved(P＜0.01).Conclusion:Reinfusion of Acu-exo can improve the survival time of hypoxic mice,and provide a basis for the basic research and application of acupuncture and preventive treatment of disease.
ElectroacupunctureExosome(Exo)HypoxiaSurvival timeEffectsAction law
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