Metabolomics study on chronic atrophic gastritis rats with liver depression and spleen deficiency syndrome
Objective:To study the biological basis of chronic atrophic gastritis(CAG)rats with liver depression and spleen deficiency syndrome from the perspective of metabolomics.Methods:Twenty-two Wistar rats were randomly divided into two groups,8 rats in the blank group and 14 rats in the model group.The blank group was given a normal diet,while the model group was given ammonia water+sodium deoxycholate+improper diet+tail clamping method to replicate the CAG rats with liver depression and spleen deficiency syndrome.The general condition of rats and their behavioral changes through open field experiments were observed;the morphological changes of gastric tissue were observed by using hematoxylin eosin(HE)staining method;liquid chromatography-mass spectrometry(LC-MS)was used to analyze the metabolomic characteristics of feces,serum,and gastric tissues in CAG rats with liver stagnation and spleen deficiency syndrome.Correlation analysis was used to construct the expression relationships of metabolites and metabolic pathways among different tissues.Results:Compared with the blank group,162 differential metabolites were identified in feces,112 differential metabolites were identified in serum,and 98 differential metabolites were identified in gastric tissue,with P＜0.05 and VIP＞1 as the threshold value.Differential metabolic pathways were screened for P＜0.05 and impact＞0,and 88 metabolic pathways were enriched in feces,three metabolic pathways were identified in order:ovarian steroidogenesis,steroid hormone biosynthesis,linoleic acid metabolism.A total of 109 metabolic pathways were enriched in serum,the top 5 identified metabolic pathways were:central carbon metabolism in cancer,protein digestion and absorption,aminoacyl tRNA biosynthesis,GABA ergic synapses,and taste transduction.A total of 112 metabolic pathways were enrichied in gastric tissue,the top 5 identified metabolic pathways were:sphingolipid signaling pathway,protein digestion and absorption,cortisol synthesis and secretion,sphingolipid metabolism and cushing syndrome.A total of 10 differential metabolites and oxidative phosphorylation metabolic pathways were screened through joint analysis.Conclusion:There are different metabolomics changes in the feces,serum,and gastric tissue of CAG rats with liver depression and spleen deficiency syndrome,and their biological basis is related to the oxidative phosphorylation.
Chronic atrophic gastritis(CAG)Liver depression and spleen deficiency syndromeMetabolomicsFecesSerumGastricAnimal modelBiological basis
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