Intervention of Qingluoyin in acute exacerbation of idiopathic pulmonary fibrosis rats by regulating the TGF-β1/Smads signal transduction pathway
Objective:To be conducted from TGF-β1/Smads signal transduction pathway,to elaborate the mechanism of the intervention of compound Qingluoyin in AE-IPF based on collateral disease theory.Methods:A total of 56 male Wistar rats were injected bleomycin through air tube for the first time and lipopolysaccharide for the second time to make AE-IPF model.The rats were divided into negative control group(Control group),remission model group(IPF group),acute exacerbation model group(AE-IPF group),low dose group(QLY-L group),medium dose group(QLY-M group),high dose group(QLY-H group)and hormone group(PNS group).The hormone group was given Prednisone acetate(3.125 mg/mL)by gavage,and the Chinese medicine group was given low,medium,high doses of Chinese herbal compound Qingluoyin(0.259,0.518,1.036 g/mL).Pulmonary tissue biopsy,arterial blood gas analysis and hydroxyproline determination were used to evaluate the pulmonary oxygen exchange capacity and the degree of pulmonary fibrosis in rats,and immunohistochemistry,Western Blot and quantitative RT-PCR were used to detect TGF-β1,TGF β R Ⅰ,TGF β R Ⅱ,Smad2,Smad4,Smad7 in rat lung tissue,to analyze TGF-β1/Smads signal transduction pathway intervenes the process of acute exacerbation of pulmonary fibrosis in rats.Results:The lung tissue texture of rats in the QLY-L,QLY-M,and QLY-H groups was softer,with fewer ecchymosis and ecchymosis compared to those in the AE-IPF groups.The edema of the lung tissue was lighter,which was basically consistent with the performance of the PNS group.Arterial blood gas analysis showed that the QLY-L,QLY-M and QLY-H groups were similar to the PNS group,but increased in the QLY-M and QLY-H group compared to the AE-IPF group(P＜0.05).The results of HYP content determination showed that the QLY-M group was similar to the PNS group,but decreased compared to the AE-IPF group(P＜0.01).There were significant differences in TGF-β1,TGF β R Ⅰ,Smad2,Smad4,and Smad7 protein and mRNA expression between the QLY-M group and QLY-H group compared to that in the AE-IPF group(P＜0.01,P＜0.05).Conclusion:Qingluoyin has a certain curative effect in the treatment of AE-IPF.It can inhibit the progression of AE-IPF by regulating TGF-β1/Smads signaling pathway.The medium and high doses can be used as reference doses for clinical application.
QingluoyinAcute exacerbation of idiopathic pulmonary fibrosis(AE-IPF)TGF-β1/Smads signal transduction pathwayIdiopathic pulmonary fibrosis(IPF)MechanismHydroxyproline(HYP)
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