Protective effect of Huazhuo Jiedu Xiaoyong Formula in mice with ulcerative colitis based on the regulation of endoplasmic reticulum stress by SIRT1
Objective:To explore the protective effect of Huazhuo Jiedu Xiaoyong Formula in mice with ulcerative colitis(UC)based on the regulation of endoplasmic reticulum stress(ERS)by silent information regulator 1(SIRT1).Methods:C57BL/6 mice were randomly divided into normal group,model group,Mesalazine group,and Huazhuo Jiedu Xiaoyong Formula high dose,medium dose and low dose group,with 10 mice in each group.The animal model of the UC was established with dextran sulfate sodium(DSS).After successful molding,Huazhuo Jiedu Xiaoyong Formula 28.68,14.34,7.17 g/kg was administered,and Mesalazine 0.45 g/kg was fed.The model group and normal group were fed with 10 mL/kg of 0.9％sodium chloride solution every day for 7 d continuously.Changes of disease activity index(DAI)score was observed,and the protein and mRNA expression levels of SIRT1,GPR78,CHOP,Caspase-3 in colon tissue of mice in each group were observed.Results:Compared with the model group,Huazhuo Jiedu Xiaoyong Formula three dose groups cowd can significantly reduce the DAI score(P＜0.05).Compared with the normal group,the mRNA and protein expression of SIRT1 in the colon tissue of the mice in the model group significantly decreased(P＜0.05).After treatment,the mRNA and protein expression of SIRT1 increased in each drug treatment group(P＜0.05).Among them,the high dose Huazhuo Jiedu Xiaoyong Formula group had the better effect,which was equivalent to the Mesalazine group.Compared with the normal group,the expression levels of GRP78,a marker of ERS,apoptosis-related molecules CHOP,and Caspase-3 in the colon tissue of the model group significantly increased(P＜0.05).After treatment,the expression of each drug treatment group significantly decreased(P＜0.05),and the high dose Huazhuo Jiedu Xiaoyong Formula group had the better effect,which was equivalent to the Mesalazine group.Conclusion:The mechanism of Huazhuo Jiedu Xiaoyong Formula in treating UC may be through up regulating the expression of SIRT1,thus inhibiting the excessive stress of endoplasmic reticulum,inhibiting the apoptosis of intestinal epithelial cells,and alleviating the damage of intestinal mucosa,so as to play the role of treating UC.
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