首页|麻花艽6个成分对类风湿性关节炎滑膜成纤维样细胞的影响及机制

麻花艽6个成分对类风湿性关节炎滑膜成纤维样细胞的影响及机制

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目的:探讨麻花艽6个成分对类风湿性关节炎滑膜成纤维样细胞(RA-FLS)的影响及机制.方法:采用CCK-8法筛选麻花艽6个成分的最佳给药浓度;流式细胞术测定最佳给药浓度干预下的细胞凋亡水平,ELISA检测细胞凋亡诱导因子(AIF)及基质金属蛋白酶(MMPs)表达水平;Western Blot法检测沉默信息调节因子1(SIRT1)、核因子-κB p65(NF-KB p65)、Bcl-2、Bax蛋白表达情况.结果:麻花艽6个成分最佳给药浓度:龙胆苦苷为150 μmol/L、马钱苷酸为150 μmol/L、獐牙菜苦苷为30 μmol/L、獐牙菜苷为150 μmol/L、异荭草素为200 μmol/L、异牡荆素为200 μ mol/L.与空白对照组比较,龙胆苦苷的促细胞凋亡作用最强(P＜0.01),马钱苷酸、獐牙菜苦苷、异荭草素次之(P＜0.01),獐牙菜苷、异牡荆素组最次(P＜0.05);各给药组能显著增加RA-FLS中AIF表达(P＜0.01,P＜0.05),显著降低RA-FLS中MMPs表达(P＜0.01,P＜0.05);各给药组能显著降低RA-FLS中NF-κB p65、Bcl-2蛋白表达(P＜0.05),显著增加SIRT1、Bax蛋白表达(P＜0.05).结论:麻花艽6个成分具有一定的抑制RA-FLS增殖活性和促凋亡作用,并能调控AIF和MMPs的表达,其潜在机制可能是通过抑制NF-κB信号通路炎症因子的释放和促进线粒体凋亡通路中诱导凋亡因子、促凋亡蛋白水平来促进RA-FLS凋亡.
Effects and its mechanism of six components of Gentiana straminea Maxim.in rheumatoid arthritis fibroblast-like synoviocytes
Objective:To investigate the effects and mechanism of six components of Gentiana straminea Maxim.on rheumatoid arthritis fibroblast-like synoviocytes(RA-FLS).Methods:The optimal concentrations of Gentiana straminea Maxim.were selected by CCK-8 method.Flow cytometry(FCM)was used to determine the expression levels of pro-inflammatory factors AIF and MMPs were detected by ELISA.The expressions of SIRT1,NF-κB p65,Bcl-2 and Bax were detected by Western Blot.Results:Optimal concentrations of six components of Gentiana straminea Maxim.:gentimacroside was 150 μmol/L,loganic acid was 150 μmol/L,swertiamarin was 30 μmol/L,sweroside was 150 μmol/L,isoorientin was 200 μmol/L,isovitexin was 200 μmol/L.Compared with the blank control group,the apoptotic effect of gentimacroside was the strongest(P＜0.01),followed by loganic acid,swertiamarin,isoorientin(P＜0.01),sweroside and isovitexin were the weakest(P＜0.05),all the treatment groups significantly increased the expression of AIF(P＜0.01,P＜0.05),and significantly decreased the expression of MMPs in RA-FLS(P＜0.01,P＜0.05),all the treatment groups could significantly reduce the expression of NF-κB p65 and Bcl-2(P＜0.05)and increase the expression of SIRT1 and Bax(P＜0.05).Conclusion:The six components of Gentiana straminea Maxim.can inhibit the proliferation activity of RA-FLS and promote apoptosis,and regulate the expression of AIF and MMPs.The potential mechanism may be to promote the apoptosis of RA-FLS by inhibiting the release of inflammatory factors in NF-κB signaling pathway and promoting the levels of apoptosis-inducing factors and pro-apoptotic proteins in mitochondrial apoptosis pathway.

Rheumatoid arthritis(RA)Synovial fibroblastsGentiana straminea Maxim.MechanismChemical componentsNF-κB signaling pathwayMitochondrial apoptosis pathway

白永峰、曹乌吉斯古楞

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内蒙古民族大学蒙医药研发工程教育部重点实验室,通辽 028000

中央民族大学民族医药教育部重点实验室,北京 100081

中国人民解放军海军军医大学药学院,上海 200433

类风湿性关节炎 滑膜成纤维细胞 麻花艽 机制 化学成分 核因子-κB信号通路 线粒体凋亡通路

国家重点研发计划中医药现代化研究重点专项内蒙古自然科学基金项目内蒙古高等学校科学技术研究项目中国博士后科学基金项目中央民族大学民族医药教育部重点实验室开放课题

2019YFC17123032020MS08025NJZZ201082017M613340KLEM-KF2019Y02

2024

中华中医药杂志
中华中医药学会

中华中医药杂志

CSTPCD北大核心
影响因子：1.135
ISSN：1673-1727
年,卷(期)：2024.39(7)
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