Improvement of radiation-induced intestinal edema by regulating water-fluid metabolism in the different administradtion methods of Guiqi Baizhu Formula
Objective:To investigate the protective effect and mechanism of prophylactic and therapeutic administration of Guiqi Baizhu Formula on radiation-induced intestinal edema.Methods:SD rats were selected to establish a radiation-induced intestinal edema model using 6 Gy X-ray abdominal irradiation,prophylactic and therapeutic drug administration was carried out.Prophylactic administration:Thirty SD rats were randomly divided into blank group,model group and Guiqi Baizhu Formula group,with 10 rats in each group;therapeutic administration:A total of 120 SD rats were randomly divided into two group(3 and 14 d after treatment),and 60 rats in each group,and randomly divided into blank,model,pure drug,low-dose,medium-dose and high-dose groups,10 rats in each group.General condition of rats was observed,dry and wet weight of colon tissues were detected,morphological changes of colon tissues were observed by HE staining,ultrastructure of colon tissues was observed by transmission electron microscopy,the expression of reactive oxygen species(ROS),hypoxia-inducible factor 1α(HIF-1α),and aquaporin 4(AQP4)was detected by immunofluorescence,and the expression of AQP4 and Na+-K+-ATPase was detected by Western Blot.Results:Compared with the blank group,the morphology and ultrastructure of colonic tissue in the model group showed significant disruption,and the water content of colonic tissue was significantly elevated(P＜0.01),the expression of ROS,HIF-1α and AQP4 was significantly elevated(P＜0.01);Na+-K+-ATPase protein expression was significantly reduced(P＜0.01).Compared with the model group,the morphological and ultrastructural disruption of colonic tissues was significantly improved after the intervention of the administration of Guiqi Baizhu formula(14 d before irradiation,3 and 14 d after irradiation),and the water content of colonic tissues was significantly reduced in all dose groups after 3 d of the therapeutic administration of Guiqi Baizhu Formula(P＜0.05,P＜0.01),after 3 and 14 d of therapeutic administration of Guiqi Baizhu Formula,the expression of ROS,HIF-1α and AQP4 was significantly reduced in all dose groups(except HIF-1 α in the low dose group after 3 d of treatment)(P＜0.01),and the expression of Na+-K+-ATPase protein was significantly elevated(P＜0.01,P＜0.05).Conclusion:Whether administered prophylactically or therapeutically,Guiqi Baizhu Formula can effectively improve radiation-induced intestinal edema,and the mechanism may be achieved by improving hypoxia and oxidative stress and regulating the expression of AQP4 and Na+-K+-ATPase.
NETL
NSTL
万方数据
