Effects of Buyang Huanwu Decoction mediated Cav1 in regulation of Notch signaling pathway on astrocyte transdifferentiation after cerebral ischemia
Objective:To investigate the mechanism of Buyang Huanwu Decoction on astrocyte transdifferentiation after cerebral ischemia based on caveolin-1(Cav1)/Notch signaling pathway.Methods:Wild(WT)mice and Cav1-/-(KO)mice were randomly divided into sham group,model group and Buyang Huanwu Decoction(BHD)group.Seven days before modeling,GFAP-EGFP adeno-associated virus was injected into the brain,MCAO method was used to create a model,and drug intervention was given for 21 days.The transdifferentiation of astrocytes in the cortical area of the ischemic side was observed,and the ischemic side was detected.Notch signaling pathway protein expression in cortical area.Results:Compared with the Sham group of the same genotype,the neurological deficit score of the model group was significantly increased,the pathological damage was obvious,and the expression of NeuN was significantly decreased(P＜0.01,P＜0.05).Compared with the model group of the same genotype,the neurological deficit score of the BHD group was significantly reduced,the pathological damage of the ischemic cortex was significantly improved,the expression of NeuN was significantly increased,the colocalization of EGFP-NeuN was significantly enhanced,and the levels of Notchl,Hesl and DLL1 proteins were significantly decreased(P＜0.01,P＜0.05).Compared with the WT-Model group,the neurological deficit score of the KO-Model group was significantly increased,the injury of the ischemic cortex was significantly aggravated,the expression of NeuN protein was significantly decreased,the expression of Hes1 protein was significantly increased(P＜0.05).Compared with the WT-BHD group,the neurological deficit score of the KO-BHD group was significantly increased,the injury of the ischemic cortex was significantly aggravated,the co-localization of EGFP-NeuN was weakened,and the expression of Notch1 protein was significantly increased(P＜0.05).Conclusion:Buyang Huanwu Decoction can promote the transfer of astrocytes to nerve cells after cerebral ischemia,and its mechanism may be related to the regulation of Notch signaling pathway.
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