Mechanism of enhancing the nourishing yin and lessen fire effect of Anemarrhenae Rhizoma after salt stir-frying by simulated processing and experimental evidence
Objective:To investigate the mechanism of chemical composition transformation and the enhancement of nourishing yin and lessen fire effect of Anemarrhenae Rhizoma(AR)after salt stir-frying.Methods:Firstly,the differences in chemical composition between AR and salt stir-fried Anemarrhenae Rhizoma(SAR)were compared by HPLC method and validated with simulated processing experiments to elucidate the chemical changes induced by concoction;secondly,a thyroxine-induced kidney yin deficiency model was established in rats to evaluate the effects of AR and SAR and the incremental ingredient mangostin in treating kidney yin deficiency.Western Blot and RT-PCR were used to detect the effects of drugs on PI3K/AKT/mTOR signaling pathway.Results:The content of nemangostin decreased and the content of mangostin increased after salt stir-frying of AR.The peaks of mangostin appeared in both the simulated salt-frying neomangostin monomer and the tablets,which proved that salt-frying could induce the conversion of neomangostin to mangostin;AR,SAR and mangostin could all treat kidney yin deficiency,but the effects of SAR and mangostin were significantly better than those of AR,which proved that mangostin was one of the active ingredients of AR in treating kidney yin deficiency.All of AR,SAR and mangostin could up-regulate the mRNA and protein expressions of PI3K,AKT and mTOR in rats with kidney-yin deficiency syndrome,and the effect of mangostin was significantly better than AR.Conclusion:Salt stir-frying of AR can increase the content of mangiferin and enhance the therapeutic effect of AR on kidney-yin deficiency syndrome by regulating the PI3K/AKT/mTOR signaling pathway.
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