Mechanism of Peiyuan Dingchuan Decoction in treating asthma airway inflammation based on the influence of IL-4/JAK1/STAT6 signal pathway on macrophage M2 polarization
Objective:To observe the effects of Peiyuan Dingchuan Decoction on airway inflammation and JAK1/STAT6 signaling pathway in asthma mice,and explore the mechanism of Peiyuan Dingchuan Decoction on airway inflammation in asthma.Methods:Forty C57BL/6 mice were randomly divided into 4 groups,with 10 mice in each group.Except for the blank group,the model group,budesonide group,and Peiyuan Dingchuan Decoction group were used to establish asthma mouse models by nebulizing and stimulating ovalbumin(OVA).The general state of each group mice was observed;the expressions of IL-4,IL-5,IL-13 in BALF and IgE levels in serum were detected by ELISA;HE staining method was used to observe the pathological and morphological changes of mouse lung tissue;The mRNA expressions of IL-4,JAK1,STAT6,Arg-1 in lung tissue were detected by qRT-PCR method;Immunohistochemical method was used to detect the expressions of Arg-1 and CD163 in lung tissue;Immunofluorescence staining was used to detect the expression of IL-4 in lung tissue.Results:Compared with the model group,the pathological characteristics of the budesonide group and the Peiyuan Dingchuan Decoction group were significantly reduced;The expressions of IL-4,IL-5,IL-13 in BALF,and IgE level in serum were significantly reduced in the budesonide group and the Peiyuan Dingchuan Decoction group showed a decrease(P＜0.05).The mRNA expressions of IL-4,JAK1,STAT6 and Arg-1 were significantly reduced in the budesonide group and Peiyuan Dingchuan Decoction group(P＜0.05).The expressions of Arg-1 and CD 163 in the lung tissue of mice in the budesonide group and Peiyuan Dingchuan Decoction group were significantly reduced(P＜0.05);The fluorescence intensity of IL-4 in the budesonide group and Peiyuan Dingchuan Decoction group was significantly reduced.Conclusion:Peiyuan Dingchuan Decoction can inhibit macrophage M2 polarization by inhibiting the activation of IL-4/JAK1/STAT6 signaling pathway,thereby achieving the therapeutic effect of asthma airway inflammation.
Peiyuan Dingchuan DecoctionChronic duration of asthmaAirway inflammationMacrophage M2 polarizationIL-4/JAK1/STAT6 signal pathway
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