补肾降压方对盐敏感性高血压大鼠肾损害及ACE2/Ang(1-7)/Mas轴的影响
Effects of Bushen Jiangya Prescription on renal damage and ACE2/Ang(1-7)/Mas axis in salt-sensitive hypertensive rats
刘巍 1熊兴江 2刘红旭 1王阶 2褚福永1
作者信息
- 1. 首都医科大学附属北京中医医院心血管科,北京 100010
- 2. 中国中医科学院广安门医院心血管科,北京 100053
- 折叠
摘要
目的:探讨补肾降压方对盐敏感性高血压大鼠(DS)血管紧张素转换酶(ACE2)/血管紧张素(Ang1-7)/Mas轴的调节作用,探讨其对高血压肾损害的治疗作用机制.方法:选用DS大鼠48只,随机分为低盐(LS)组、高盐(HS)组、缬沙坦(Va)组和补肾降压(BS)组,每组12只,LS组喂以0.4％低盐饲料,其余3组喂以8％高盐饲料,不同饲料喂养3周后每日按照2 mL/100 g灌胃,连续给药8周.给药前后测量收缩压,酶法检测大鼠尿微量白蛋白(mAlb)、β 2-微球蛋白(β 2-MG)及24 h尿蛋白定量水平及血浆中AngⅡ、Ang(1-7)的含量,苏木素-伊红(HE)染色肾脏组织,RT-PCR法及Western Blot法分别检测肾脏ACE2、Mas mRNA及蛋白表达.结果:给药后,与LS组比较,其余各组收缩压均明显升高,尿mAlb、尿β2-MG及24 h尿蛋白定量升高,血浆AngⅡ升高,Ang(1-7)降低,肾脏ACE2、Mas蛋白及mRNA表达显著降低(P＜0.05,P＜0.01).与HS组比较,Va及BS组各时间点收缩压降低,尿mAlb、尿β2-MG及24h尿蛋白定量降低,血浆AngⅡ降低,Ang(1-7)升高、肾脏ACE2、Mas蛋白及mRNA表达升高(P＜0.05,P＜0.01).结论:补肾降压方平稳降压,改善尿蛋白的作用,其作用机制可能与调节ACE2/Ang(1-7)/Mas轴从而拮抗肾素-血管紧张素-醛固酮系统(RAAS)系统活性相关.
Abstract
Objective:To investigate the effects of Bushen Jiangya Prescription on angiotensin converting enzyme(ACE2)/angiotensin(Ang)(l-7)/Mas axis in Dahl salt-sensitve rats(DS),and to explore the mechanism of its prevention and treatment of renal damage in hypertension.Methods:Forty-eight DS rats were randomly divided into low salt(LS)group,high salt(HS)group,valsartan(Va)group and Bushen Jiangya(BS)group,with 12 rats in each group.LS group was fed with 0.4％low salt diet,HS group,Va group and BS group were fed with 8％high salt diet.After 3 weeks of different diets,2 mL/100 g was given intragastric administration for 8 consecutive weeks.Blood pressure was measured once before administration.Urine microalbumin(mAlb),β 2-microglobulin(β 2-MG)and 24h urine protein were detected by enzyme method,plasma Ang Ⅱ,Ang(1-7)content were detected by ELISA method,kidney tissue was stained with hematoxylin-eosin(HE).The mRNA expressions of ACE2 and Mas in kidney were detected by RT-PCR,and the protein expressions of ACE2 and Mas in kidney were detected by Western Blot.Results:After administration,compared with LS group,systolic blood pressure,urine mAlb,urine β 2 and 24h urine protein were increased,plasma Ang Ⅱ level was increased,Ang(1-7)level was decreased,ACE2,Mas protein and mRNA expression were decreased(P＜0.05,P＜0.01).Compared with HS group,Va and BS groups decreased systolic blood pressure,urine mAlb,urine β 2 and 24h urine protein,plasma Ang Ⅱ level decreased,plasma Ang(1-7)level increased,ACE2,Mas protein and mRNA expression in kidney increased(P＜0.05,P＜0.01).Conclusion:Bushenjiangya Prescription can stabilize blood pressure and improve urinary protein,and its mechanism may be related to antagonizing the activity of renin-angiotensin-aldosterone system(RAAS)by regulating ACE2/Ang(1-7)/Mas axis.
关键词
盐敏感性高血压/补肾降压方/高血压肾损害/血管紧张素转换酶2/血管紧张素(1-7)/Mas轴Key words
Salt-sensitive hypertension/Bushenjiangya Prescription/Hypertensive renal damage/Angiotensin converting enzyme 2/angiotensin(1-7)/Mas axis引用本文复制引用
基金项目
国家自然科学基金项目(82204997)
国家中医药管理局中医药循证能力建设项目(2019XZZX-XXG001)
第六批北京市级中医药专家学术经验继承工作项目()
出版年
2024
国家科技期刊平台
NSTL
万方数据