Effects of Bushen Jiangya Prescription on renal damage and ACE2/Ang(1-7)/Mas axis in salt-sensitive hypertensive rats
Objective:To investigate the effects of Bushen Jiangya Prescription on angiotensin converting enzyme(ACE2)/angiotensin(Ang)(l-7)/Mas axis in Dahl salt-sensitve rats(DS),and to explore the mechanism of its prevention and treatment of renal damage in hypertension.Methods:Forty-eight DS rats were randomly divided into low salt(LS)group,high salt(HS)group,valsartan(Va)group and Bushen Jiangya(BS)group,with 12 rats in each group.LS group was fed with 0.4％low salt diet,HS group,Va group and BS group were fed with 8％high salt diet.After 3 weeks of different diets,2 mL/100 g was given intragastric administration for 8 consecutive weeks.Blood pressure was measured once before administration.Urine microalbumin(mAlb),β 2-microglobulin(β 2-MG)and 24h urine protein were detected by enzyme method,plasma Ang Ⅱ,Ang(1-7)content were detected by ELISA method,kidney tissue was stained with hematoxylin-eosin(HE).The mRNA expressions of ACE2 and Mas in kidney were detected by RT-PCR,and the protein expressions of ACE2 and Mas in kidney were detected by Western Blot.Results:After administration,compared with LS group,systolic blood pressure,urine mAlb,urine β 2 and 24h urine protein were increased,plasma Ang Ⅱ level was increased,Ang(1-7)level was decreased,ACE2,Mas protein and mRNA expression were decreased(P＜0.05,P＜0.01).Compared with HS group,Va and BS groups decreased systolic blood pressure,urine mAlb,urine β 2 and 24h urine protein,plasma Ang Ⅱ level decreased,plasma Ang(1-7)level increased,ACE2,Mas protein and mRNA expression in kidney increased(P＜0.05,P＜0.01).Conclusion:Bushenjiangya Prescription can stabilize blood pressure and improve urinary protein,and its mechanism may be related to antagonizing the activity of renin-angiotensin-aldosterone system(RAAS)by regulating ACE2/Ang(1-7)/Mas axis.
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