Mechanism of Fangji Huangqi Decoction on alleviating collagen-induced arthritis in rats by inhibiting the Notch1 pathway
Objective:To explore the mechanism of Fangji Huangqi Decoction(FHD)on alleviating collagen-induced arthritis(CIA)in rats through Notch1 pathway.Methods:The rat model of collagen-induced arthritis(CIA)was established by injection of type Ⅱ bovine collagen solution mixed with complete/incomplete Freund's adjuvant.The low,middle and high dose FHD groups were treated with 2.2,4.4,8.8 g·kg-1·d-1 FHD respectively.The arthritis score and paw swelling were measured every week.The pathological morphology of joint tissue was observed by Masson staining,the expression of interleukin(IL)-1 β,IL-4,IL-10 and IL-17 in serum was detected by ELISA.The mRNA levels of Notch1,Jagged1 and Hes1 in joint and spleen were detected by qRT-PCR,Notch1,Jagged1,Hes1 and Notch intracellular domain(NICD)protein in joint and spleen were detected by Western Blot,and the positive areas of Notch1,Jagged1,Hes1 and NICD in joint were evaluated by immunohistochemistry.Results:Compared with CIA group,in the middle and high dose FHD groups,arthritis scores and foot paw swelling were decreased(P＜0.01),joint destruction reduced,IL-1 β and IL-17 were decreased(P＜0.01),and IL-4 and IL-10 were increased(P＜0.01),the protein and mRNA expressions of Notch1,Jagged1,Hes1,and the protein expression of NICD in the spleen and joints were down-regulation(P＜0.01),the positive area of Notch1,Jagged1,Hes1 and NICD were decreased(P＜0.01,P＜0.05).Conc1usion:FHD restore the balance of inflammatory cytokine network and play an anti-arthritis effect by inhibiting Notchl signal pathway.
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