首页|芡壳多酚通过调节NLRP3/IL-1β信号通路拮抗肺癌的免疫抑制研究

芡壳多酚通过调节NLRP3/IL-1β信号通路拮抗肺癌的免疫抑制研究

Study on immunosuppression of antagonizing lung cancer based on regulating NLRP3/IL-1β signaling pathway by Euryale shell polyphenols

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目的:探讨芡壳多酚通过炎症因子抑制肺癌的作用及其机制.方法:通过腋下注射LLC细胞建立小鼠皮下瘤模型,分别给予不同剂量的芡壳多酚(6、12 g/kg)和5-氟尿嘧啶(20 mg/kg).对肿瘤组织进行HE染色、TUNEL凋亡染色、蛋白质印迹和免疫组织化学分析,评估小鼠肿瘤的形态、炎症环境、CD4+和CD8+T细胞浸润以及NLRP3炎症体相关蛋白的表达水平.结果:芡壳多酚可以有效抑制肿瘤增殖并促进细胞凋亡(P＜0.01,P＜0.05),改善肿瘤组织中炎症环境,增加CD4+、CD8+T细胞浸润(P＜0.01,P＜0.05),提高小鼠抗肿瘤免疫力,下调NLRP3、Cleaved-caspase-1/Caspase-1、ACS和IL-1β 的蛋白表达(P＜0.01).结论:芡壳多酚可能通过NLRP3/IL-1β信号阻碍NLRP3炎性体的激活和炎症因子的释放,增加免疫细胞募集,拮抗肺癌的免疫抑制,从而抑制小鼠皮下肿瘤的作用.
Objective:To investigate the inhibitory effect of Euryale shell polyphenols on lung cancer through inflammatory factors and its mechanism.Methods:LLC cells were injected under the armpit of mice to establish subcutaneous tumor model.Different doses of Euryale shell polyphenols(6,12 g/kg)and 5-Fluorouracil(20 mg/kg)were given respectively.HE staining,TUNEL apoptosis staining,Western Blot and immunohistochemical analysis were performed to evaluate tumor morphology,inflammatory environment,CD4 and CD8+T cell infiltration,and NLRP3 inflammasome related protein expression.Results:Euryale shell polyphenols inhibited tumor proliferation and promoted cell apoptosis(P＜0.01,P＜0.05),improved the inflammatory environment in tumor tissues,and increased the infiltration of CD4+and CD8+T cells(P＜0.01,P＜0.05),improved anti-tumor immunity in mice,and decreased NLRP3,Cleaved-caspase-l/Caspase-1,ACS and IL-1 β protein expression(P＜0.01).Conclusion:Euryale shell polyphenols can inhibit the activation of NLRP3 inflammasome and the release of inflammatory factors through NLRP3/IL-1 β signaling pathway,increase the recruitment of immune cells,antagonize the immunosuppression of lung cancer,and thus inhibiting the effect of subcutaneous tumors in mice.

Euryale shell polyphenolsImmunosuppressionNLRP3/IL-1β signaling pathwayLLC cellsInflammatory microenvironment

单鑫、向东阳、陈璟、吕志阳

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南京中医药大学翰林学院药学院,泰州 225300

南京中医药大学中西医结合学院,南京 210046

芡壳多酚 免疫抑制 NLRP3/IL-1β信号通路 LLC细胞 炎症微环境

泰州市科技支撑计划(农业)项目江苏省高校自然科学基金项目江苏省高校自然科学基金项目江苏省中医药管理局项目

TN20212321KJB36001822KJB360014YB2020108

2024

中华中医药杂志
中华中医药学会

中华中医药杂志

CSTPCD北大核心
影响因子：1.135
ISSN：1673-1727
年,卷(期)：2024.39(8)