Study on the extraction and purification process of the anti-inflammatory components from Swertia mussotii Franch.
Objective:To establish a rapid and efficient extraction and purification process for the anti-inflammatory component of iridoid glycosides from Swertia mussotii Franch..Methods:Single factor experiment,response surface methodology and Box-Behnken were used to optimize the extraction parameters of three iridoid glycosides,swertiamarin,gentiopicroside and sweroside.The macroporous resin column packing was selected,and the iridoid glycosides were purified and enriched,and the anti-inflammatory activity in vitro was studied.Results:The optimal extraction parameters of the three iridoid glycosides from Swertia mussotii Franch.were as follows:extraction times 2 times,extraction time 60 min,liquid-solid ratio 46 mUg,ethanol concentration 73％,extraction temperature 50 ℃.The results showed that the extraction rate of the three iridoid glycosides in Swertia mussotii Franch..was 0.47％.The iridoid glycosides were enriched and purified by DM301 macroporous resin column.The total content of iridoid glycosides in the enriched components was 62.30％.Conclusion:The optimum extraction and purification process of iridoid glycosides from Swertia mussotii Franch.was established,which improved the content of iridoid glycosides and the utilization rate of medicinal materials.
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