目的:研究滇产迷迭香中鼠尾草酸(CA)最佳提取条件和最佳采收期,为滇产迷迭香质量检测和应用提供参考.方法:采用反相高效液相色谱法,色谱柱:Elite-C18(4.6 mm × 250 mm,5 μ m),流动相:甲醇-0.5％磷酸溶液,流速:0.8mUmin,检测波长:283 nm,柱温:30℃.对CA供试品溶液的制备方法进行研究,采用外标法对滇产迷迭香不同采收期CA含量进行测定.结果:对CA的提取条件进行研究得到最佳提取条件,溶剂为90％乙醇,粒径为100目,料液比为1:60,超声2次,超声时间5 min,超声功率为120 W;在此基础上测定了滇产迷迭香不同季节中CA的含量,结果显示CA在秋冬季节的含量较高,春夏两季相对较低;10月份含量达到最高,可达6.02％.结论:为迷迭香中CA含量测定提供一种高效的供试品液制备方法,并为滇产迷迭香的采收提供参考.
Changes in the content of carnosic acid at different harvesting periods of Rosmarinus oficinalis L.from Yunnan and the effects of extraction conditions on content determination
Objective:To study the optimal extraction conditions and best harvesting period of carnoic acid(CA)in Rosmarinus officinalis L.from Yunnan Province,providing references for the quality inspection and application of Yunnan Rosemary.Methods:The reversed phase high performance liquid chromatography(RP-HPLC)method was applied in this study.The chromatographic conditions were as following:Elite-C18 column(4.6 mmx250 mm,5 μm),mobile phase:methanol-0.5％phosphate acid solution,flow rate:0.8 mUmin,detection wavelength:283 nm,column temperature:30 ℃.The preparation method of CA test solution was studied and the CA content of Rosmarinus officinalis L.in different harvest periods in Yunnan was determined by external standard method.Results:The optimum extraction conditions for CA were obtained by studying the extraction conditions,with 90％ethanol as solvent,100 mesh particle size,1:60 material-to-liquid ratio,ultrasonic extraction twice,ultrasonic time of 5 min,and ultrasonic power of 120 W.Based on this,the content of CA in Rosmarinus officinalis L.from Yunnan in different seasons was determined,and the results showed that the content of CA was higher in autumn and winter,and relatively lower in spring and summer.The content reached the highest level in October,up to 6.02％.Conclusion:This study provides an efficient method for the preparation of test sample solution for the determination of CA content in rosemary and a reference for the harvest of Rosmarinus officinalis L.from Yunnan.
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