Effects of moxa smoke on cognitive ability and hypothalamic-pituitary-gonadal axis hormones in APP/PS1 mice
Objective:To observe the effects of moxa smoke on cognitive ability and hypothalamic-pituitary-gonadal axis hormone levels in APP/PS1 mice.Methods:Twenty-seven male APP/PS1 mice were randomly divided into model group,rosemary essential oil group(hereinafter referred to as essential oil group)and moxa combustion products group(hereinafter referred to as moxa smoke group),with nine mice in each group.Nine C57BL/6 mice with the same genetic background were used as the blank group.The essential oil group and the moxa smoke group were respectively treated with 1％rosemary essential oil and 10-15 mg/m3 moxa smoke for sniffing intervention.Mice in the blank group and the model group were only grasped and fixed.After 12 weeks,the Morris water maze test was used to detect the learning and memory ability of the mice.ELISA was used to detect serum levels of gonadotropin releasing hormone(GnRH),luteinizing hormone(LH),follicle stimulating hormone(FSH)and testosterone(T).Congo red staining was used to observe the deposition of β-amyloid protein(A β)in the prefrontal cortex of mice.Results:Compared with model group,the escape latency of essential oil group on days 3-5 and moxa smoke group on days 4-5 shortened significantly(P＜0.05).The number of crossing the platform and swimming time in the platform area were significantly increased(P＜0.05).The contents of LH and FSH were significantly decreased(P＜0.05,P＜0.0 1),T content significantly increased(P＜0.05).A β deposition was reduced in the prefrontal cortex;The proportion of swimming distance in the platform area of the essential oil group was significantly increased(P＜0.05),and the GnRH content was significantly decreased(P＜0.01).Conclusion:Moxa smoke can effectively reduce the deposition of A β in the brain of APP/PS1 mice and improve the cognitive ability by correcting the disorder of HPG axis hormones.
MoxibustionAlzheimer diseaseMoxa smokeHormoneCognitive abilityHypothalamus-pituitary-gonad axisLearning and memory
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