Effects of electroacupuncture 'Neiguan(PC 6)-Zhongwan(RN 12)'on gastric motility and related brain gut peptides in functional dyspepsia rats
Objective:To observe the effects of electroacupuncture Neiguan(PC 6),Zhongwan(RN 12)and Neiguan-Zhongwan on gastric motile and braingutpeptide in functional dyspepsia(FD)rats.Methods:A total of 56 SPF grade male SD rats were randomly divided into normal group(n=16)and modeling group(n=40).The FD rat model was prepared by using 0.1％sucrose iodoacetamide solution and modified small platform method.After confirming the successful preparation of the model,the remaining 32 rats in the modeling group were randomly divided into model group,Neiguan group,Zhongwan group and Neiguan-Zhongwan group,with 8 rats in each group.The above intervention groups were respectively subjected to electroacupuncture intervention of bilateral Neiguan(PC 6)and Zhongwan(RN 12)and Neiguan(PC 6),and Zhongwan(RN 12),with continuous wave,frequency 2 Hz,current intensity 1 mA,30 min/d for 7 days.The gastric emptying rate was measured after the intervention.The histopathologic changes of gastric antrum were observed by HE staining,and the content of 5-HT in serum was detected by ELISA.The expression levels of gastrin(GAS),motilin(MTL),calcitonin gene-related peptide(CGRP)in serum and substance P(SP),cholecystokinin(CCK)and Ghrelin in antrum tissues were detected by Western Blot.Results:Under light microscope,the mucosa of gastric antrum tissue was intact,the glands were arranged in order,and no obvious inflammatory infiltration was observed in gastric mucosa.Compared with model group,gastric emptying rate in Zhongwan group and Neiguan-Zhongwan group was significantly increased(P＜0.0 1),and serum 5-HT content was significantly decreased(P＜0.05).The protein expressions of GAS,MTL and Ghrelin in Neiguan group,Zhongwan group and Neiguan-Zhongwan group were significantly increased(P＜0.05,P＜0.01),while the protein expression of SP was significantly decreased(P＜0.05,P＜0.0l).The expression level of CGRP protein in Neiguan-Zhongwan group was significantly decreased(P＜0.05),and the expression level of CCK protein was significantly increased(P＜0.05).The protein expression levels of GAS and MTL in Neiguan-Zhongwan group were significantly higher than those in Zhongwan and Neiguan groups(P＜0.01,P＜0.05),and the protein expression levels of SP in Neiguan-Zhongwan group were significantly lower than those in Neiguan group(P＜0.05).The expression of Ghrelin protein in Neiguan-Zhongwan group was significantly higher than that in Zhongwan group(P＜0.01),the expression of MTL and Ghrelin protein in Zhongwan group was significantly higher than that in Neiguan group(P＜0.0l,P＜0.05).Conclusion:Electroacupuncture Neiguan-Zhongwan can improve gastric motility disorder in FD rats,relieve dyspeptic symptoms in rats,and reduce the level of brain and intestinal peptides such as CCK and CGRP,which is obviously better than Zhongwan and Neiguan alone,which may be related to the coordination of the two points to regulate the function of brain and intestinal axis,thus affecting the secretion of brain and intestinal peptides.
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