Bear bile powder alleviates colitis by improving intestinal epithelial barrier function via inhibiting MLCK/pMLC2 signaling pathway
Objective:To explore the therapeutic effect and mechanism of bear bile powder on colitis.Methods:Colitis was induced in mice by making them freely drink water solution containing 3％dextran sulfate sodium salt(DSS).After successful modeling,bear bile powder solutions of different concentrations(150,300,600 mg/kg)were given for seven consecutive days,and Disease activity index(DAI)were performed.Measurement of colon length;HE staining was used to observe the pathological changes of colon.Alcian blue staining was used to observe the number of mucins.The integrity of intestinal barrier was detected by FITC-Dextran.The expression of Occludin,E-cadherin and Dsg2 were observed by immunofluorescence staining and Western Blot.Detection of MLCK/pMLC2 signaling pathway protein by Western Blot.Results:Compared with the control group,mice in the model group showed a significant decrease in body weight,a rise in DAI score and histological score,and a shortened colon(P＜0.01).Low and medium doses of bear bile powder alleviated the decrease in body weight,reduced DAI scores,protected the integrity of mice colon tissues,alleviated the shortening of the colon,and reduced histological scores(P＜0.01),increased the expression of the proteins Occludin,Dsg2,and E-cadherin(P＜0.01),and reduced the expression of the MLCK protein,and reduced the pMLC2/MLC2 protein ratio(P＜0.01).Conclusion:Bear bile powder plays an anti-colitis role by inhibiting MLCK/pMLC2 signaling pathway and protecting cell connexin repairing intestinal barrier.
Bear bile powderColitisMucinIntestinal barrierMLCK/pMLC2 pathway
NETL
NSTL
万方数据
