Effects of Zhuyu Pills on intestinal flora structure of atherosclerosis mice based on 16S rDNA sequencing
Objective:To observe the effect of Zhuyu Pills on intestinal flora of ApoE-/-atherosclerosis(AS)mice.Methods:The mouse model of AS was established by feeding with a high-fat diet for 12 weeks.The 50 successful modeled ApoE-/-mice were randomly divided into model group,low,medium and high dose groups of Zhuyu Pills and atorvastatin calcium group,with 10 mice in each group.Ten C57BL/6J mice were selected as normal group.After modeling,normal group and model group were given equal volume of sterile distilled water by gavage,low,medium and high dose groups were given 130.54,261.08 and 552.16 mg/kg by gavage,respectively,and atorvastatin calcium group was given 10.40 mg/kg atorvastatin calcium suspension by gavage,all of which were given gavage for a total of 12 weeks.The body weight and food intake of mice were detected.Hematoxylin-eosin(HE)staining was used to observe the pathological changes of aorta in mice.The levels of oxidized low-density lipoprotein(ox-LDL),monocyte chemotactic egg-1(MCP-1),vascular cell adhesion-1(VCAM-1)and intercellular adhesion molecule-1(ICAM-1)in serum were detected by enzyme-linked immunosorbent assay(ELISA).16S rDNA technique was used to detect the diversity of Alpha and Beta,and species composition of intestinal flora in mouse feces.Results:Compared with normal group,body weight and food intake of mice in model group decreased.HE staining showed large area thickening of the aortic intima.Serum ox-LDL,MCP-1,VCAM-1 and ICAM-1 levels were increased significantly(P＜0.01).Chao1 index,Ace index and observed species index decreased(P＜0.01).The relative abundance of Verrucomicrobiota,Dubosiella and Lactobacillus increased(P＜0.01),while the relative abundance of Bacteroidota,Actinobacteria,Ligilactobacillus,Faecalibaculum and Blautia decreased significantly(P＜0.01).Compared with the model group,the body weight and food intake of each administration group increased.HE staining showed that with the increase of the concentration of Zhuyu Pills,the plaque enrichment area decreased gradually.Serum ox-LDL,MCP-1,VCAM-1 and ICAM-1 were decreased significantly(P＜0.01);Chao1 index,Ace index and observed species index were increased significantly(P＜0.01).The relative abundance of Bacteroidota,Proteobacteria,Faecalibaculum and Blautia increased significantly(P＜0.01),while Firmicutes,Actinobacteria,Verrucomicrobiota,Dubosiella,Ligilactobacillus and Lactobacillus decreased significantly(P＜0.01).Conclusion:Zhuyu Pills can significantly inhibit the pathological changes of aorta in AS mice,regulate the relative abundance of beneficial and harmful bacteria in intestinal flora,correct the flora disorder,and thus reduce the inflammatory response of aorta.
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