Exploration on the anti-inflammatory and healing effects of ulcer oil on chronic wounds based on NLRP3/Caspase-1 signaling pathway
Objective:To observe the wound healing effect of ulcer oil on rabbits with chronic skin ulcers and the expression of nucleotide-binding oligodomain-like receptor protein 3(NLRP3),Caspase-1,and nuclear factor-kappa B(NF-κB)in the wound tissue.Methods:A total of 60 New Zealand white rabbits were randomly divided into blank group(n=15)and molded group(n=45).The chronic skin ulcer model was replicated by'hormonal intervention-skin defect-bacterial infection'compound factor method.After success,it was randomly divided into model control group,ethacridine group and ulcer oil group.15 mice in each group were treated with 0.9％sodium chloride solution,ethacridine lactate and ulcer oil for 21 d.Wound area,tissue proliferating cell nuclear antigen(PCNA),NLRP3,Caspase-1,NF-κB p65 protein expression,and blood routine,liver function,kidney function and other safety indicators were detected at intervention 7,14 and 21 d time points.Results:At 7 days of intervention,compared with the model control group and the lactate ethacridine group,the ulcer oil group showed a significant reduction in wound area(P＜0.05);Compared with the blank group,the model control group showed a significant decrease in PCNA protein expression(P＜0.01)and a significant increase in NF-κB p65 protein expression(P＜0.0l);Compared with the model control group and the lactate ethacridine group,the expression of PCNA protein in the ulcer oil group was significantly increased(P＜0.01);Compared with the model control group,the NF-κB p65 protein expression was significantly reduced in the lactate ethacridine group and ulcer oil group(P＜0.01).At 14 days of intervention,compared with the model control group and the lactate ethacridine group,the ulcer oil group showed a significant decrease in wound area(P＜0.01)and a significant increase in PCNA protein expression(P＜0.05);Compared with the model control group,the expression of NLRP3 and NF-κB p65 proteins was significantly reduced in the lactate ethacridine group and ulcer oil group(P＜0.01,P＜0.05).At 21 days of intervention,compared with the model control group,both the lactate ethacridine group and the ulcer oil group showed a significant reduction in wound area(P＜0.01);Compared with the blank group,the model control group showed a significant decrease in PCNA protein expression(P＜0.01)and a significant increase in NF-κB p65 protein expression(P＜0.01);Compared with the model control group and the lactate ethacridine group,the expression of PCNA protein in the ulcer oil group was significantly increased(P＜0.01);Compared with the model control group,the NF-κB p65 protein expression was significantly reduced in the lactate ethacridine group and ulcer oil group(P＜0.05,P＜0.01).Conclusion:Ulcer oil may reduce wound inflammation by downregulating the expression of key proteins in the NLRP3/Caspase-1 signaling pathway,especially NLRP3 and NF-κB p65 proteins,while promoting wound tissue cell proliferation,accelerating wound healing,and having good safety during the intervention process.
NETL
NSTL
万方数据
