Establishment and evaluation of a mouse model combining acne and damp-heat syndrome of spleen and stomach
Objective:To establish a mouse model combining acne and damp-heat syndrome of spleen and stomach,guided by clinical manifestations,through the integration of pathogenesis and pathophysiology modeling methods with modeling methods for spleen and stomach damp-heat syndrome.Methods:The model combining acne and damp-heat syndrome was created by combining intradermal injection of Cutibacterium acnes(C.acnes).with a high-fat and high-sugar diet(internal damp-heat method).The model was further verified by the administration of heat-clearing and dampness-resolving Chinese herbal formulations.Various parameters,including body weight,activity level,rectal temperature,and bowel habits,were compared to evaluate the degree of spleen and stomach damp-heat.Skin microscopy and Laser Speckle Contrast Imaging were used to observe changes in the acne area on the backs of mice.HE staining was employed to observe inflammatory changes in the skin and gastric mucosal tissues,and oil red O staining was used to observe sebum secretion.Immunohistochemistry was utilized to observe the expression levels of IGF-1R,PPAR-γ,SREBP-1,and AR in mice.Results:Compared with the blank group and the C.acnes group,mice in the spleen and stomach damp-heat model group exhibited reduced activity(P＜0.05),delayed response,weight loss,yellowish and soft stools,and infiltration of inflammatory cells in the intrinsic and submucosal layers of the gastric mucosa.The acne lesions were more severe,and the local blood flow in the acne area was more pronounced(P＜0.01,P＜0.05),with upregulation of relevant inflammatory factors(P＜0.0l,P＜0.05).In addition,mice in the spleen and stomach damp-heat model group had enlarged sebaceous glands(P＜0.01)and increased sebum secretion(P＜0.01).After intervention with traditional Chinese medicine,these changes were alleviated.Compared with the blank group and the C.acnes group,the expression of IGF-1R in the epidermis of mice in the spleen and stomach damp-heat model group increased(P＜0.01),and the positive expression of PPAR-γ,SREBP-1,and AR in the sebaceous glands significantly increase(P＜0.01).After traditional Chinese medicine intervention,the positive expression of PPAR-γ,SREBP-1,and AR in the sebaceous glands of mice decreased(P＜0.01).Conclusion:Feeding mice with a high-fat and high-sugar diet to simulate human spleen and stomach damp-heat,combined with intradermal injection of C.acnes to simulate the pathological process of acne inflammation,comprehensively forms a reliable mouse model of acne and spleen-stomach damp-heat.This model can promote research on the pathological mechanisms of acne and the evaluation of the efficacy of traditional Chinese medicine.
AcneSpleen and stomach damp-heat syndromeDisease-syndrome combinationModelInflammationSebumCutibacterium acnes
NETL
NSTL
万方数据
