Abdominal acupuncture regulates the metabolism of water,salt and sugar to treat chronic psychological stress lung cancer bearing mice experiment
Objective:To confirm that the progression of lung cancer is related to chronic psychological stress,and abdominal acupuncture can regulate glucose metabolism and water and salt metabolism to calm chronic psychological stress trauma and inhibit lung cancer.Methods:Male C57BL/6J mice aged 7-8 months were randomly divided into 4 groups according to body weight,with 16 mice in each group.The animals were divided into the following groups:control group,mice bearing tumor,feeding and drinking freely without any intervention;Chronic psychological stress group,mice bearing tumor,by grasping the back skin of mice every other day for 10 s to achieve chronic psychological stress effect,the main observation index is hair color lightening;Chronic psychological stress+abdominal acupuncture group(referred to as abdominal acupuncture group):mice bearing tumor,hold the back skin of mice and select the abdominal Renmai Qihai,Guanyuan and Zhongwan points,spleen meridian Dahorizontal(bilateral),Fujie(bilateral),Fushe(bilateral),needle one point each time,each acupuncture point lifting and twisting for 10 s,flat supplement and flat diarrhea,once every other day;Chronic psychological stress plus cyclophosphoxide group(cyclophosphoxide group for short),mice bearing tumor,hold the back skin of mice and intraperitoneal injection of cyclophosphoxide,50 mg/kg dose,once every 5 days.After 1 week of adaptive feeding and 1 week of injection or cyclophospholide,all mice were inoculated with 0.2 mL(5×106/mL)Lewis lung cancer cells under the armpit of the right forelimb,and the tumors erupted after continued injection or administration for 1 week,and the tumors were taken and weighed after continued injection or administration for 1 week.The mRNA and protein expressions of glucose metabolism-related proteins AKT2 and GSK3 β,water and salt metabolism-related proteins AQP1 and ATP1A1,and anti-apoptosis protein MCL1 were detected.SPSS 22.0 statistical software was used to calculate and compare the detection data of each group.Results:The local hair color of mice under chronic psychological stress became lighter,and the tumor growth in the abdominal acupuncture group was significantly slower than that in the chronic psychological stress group and the control group(P＜0.01).The tumor proteins related to glucose metabolism AKT2 and GSK3 β,and the proteins related to water and salt metabolism AQP1 and ATP1A1 in the abdominal acupuncture group were significantly slower than that in the chronic psychological stress group and the control group.The mRNA and protein expression of anti-apoptosis protein MCL1 were significantly decreased compared with chronic psychological stress group(P＜0.01).Conclusion:The development of lung cancer is related to chronic psychological stress.Abdominal acupuncture can relieve the damage caused by chronic psychological stress by regulating glucose metabolism pathway AKT2/GSK3 β,water and salt metabolity-related proteins AQP1 and ATP1A1,reducing the expression of anti-apoptosis protein MCL1 and inhibiting lung cancer.
Chronic psychological stressLung cancerTumorAbdominal acupunctureAcupunctureTraditional Chinese medicine
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NSTL
万方数据
