首页|基于TLR4/NF-κB信号通路探讨柴胡陷胸汤含药血清对脂多糖诱导人胃黏膜上皮GES-1细胞的作用机制

基于TLR4/NF-κB信号通路探讨柴胡陷胸汤含药血清对脂多糖诱导人胃黏膜上皮GES-1细胞的作用机制

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目的:探讨柴胡陷胸汤含药血清对脂多糖(LPS)诱导人胃黏膜上皮GES-1细胞Toll样受体4(TLR4)/核因子κB(NF-κB)信号通路的影响,以揭示该方治疗慢性胃炎可能的作用机制.方法:GES-1细胞分为4组:空白组:GES-1细胞+正常大鼠血清;模型组:GES-1细胞+LPS诱导+正常大鼠血清;柴胡陷胸汤组:GES-1细胞+LPS诱导+柴胡陷胸汤含药血清;吡咯烷二硫代甲酸铵(PDTC)组:GES-1细胞+LPS诱导+PDTC.运用MTT检测LPS的细胞毒性;运用CCK8法检测细胞活性;运用ELISA法检测细胞炎症因子白细胞介素(IL)-1β、IL-6水平;运用Western Blot法检测TLR4、NF-κB、p-NF-κB、核苷酸寡聚结合域样受体蛋白3(NLRP3)蛋白表达水平.结果:与空白组比较,模型组细胞炎症因子IL-1β、IL-6水平显著升高(P＜0.01),与模型组比较,给予柴胡陷胸汤含药血清作用后,GES-1细胞IL-1β、IL-6水平显著降低(P＜0.01);与空白组比较,模型组GES-1细胞TLR4、NLRP3蛋白表达及NF-κB磷酸化水平显著升高(P＜0.01),与模型组比较,柴胡陷胸汤组GES-1细胞TLR4、NLRP3蛋白表达及NF-κB磷酸化水平显著降低(P＜0.01).结论:柴胡陷胸汤可通过TLR4/NF-κB信号通路抑制LPS诱导的IL-1β和IL-6分泌,减轻GES-1细胞损伤.
Mechanism of Chaihu Xianxiong Decoction-containing serum on human gastric epithelial GES-1 cells induced by lipopolysaccharide based on TLR4/NF-κB signaling pathway
Objective:To investigate the effect of serum containing Chaihu Xianxiong Decoction(CHXXD)on TLR4/NF-κB signaling pathway induced by lipopolysaccharide(LPS)in human gastric epithelial GES-1 cells,and to reveal the possible mechanism of CHXXD in treating chronic gastritis.Methods:GES-1 cells were divided into the following four groups:blank group:GES-1 cells+normal rat serum;model group:GES-1 cells+LPS induction+normal rat serum;CHXXD group:GES-1 cells+LPS induction+serum with CHXXD;PDTC group:GES-1 cells+LPS induction+PDTC.Cell morphology was observed and cytotoxicity of LPS was detected by MTT;the effect of drug serum on GES-1 cell viability was detected by CCK8;the levels of IL-1β and IL-6 in each group were detected by ELISA;the protein expressions of TLR4,NF-κB,phosphorylated NF-κB and NLRP3 in all groups were detected by Western Blot.Results:Compared with the blank group,the levels of inflammatory cytokines IL-1β and IL-6 in model group were significantly increased(P＜0.01).Compared with the model group,the levels of IL-1β and IL-6 in GES-1 cells were significantly decreased after the combined action CHXXD drug serum(P＜0.01).Compared with the blank group,the protein levels of TLR4,phosphorylated NF-κB and NLRP3 in the model group were significantly increased(P＜0.01).Compared with the model group,the levels of TLR4,phosphorylated NF-κB and NLRP3 protein in GES-1 cells were significantly decreased after combined treatment with CHXXD(P＜0.0l).Conclusion:CHXXD can inhibit the secretion of IL-1β and IL-6 induced by LPS through TLR4/NF-κB signaling pathway,and alleviate the injury of GES-1 cells.

TLR4/NF-κB signaling pathwayChaihu Xianxiong DecoctionLipopolysaccharideHuman gastric epithelial GES-1 cellsMechanism

林云崖、刘松林、明浩、林玲、杨宇、陈雨、岳滢滢、许乐思、周贤

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湖北中医药大学,武汉 430065

江汉大学,武汉 430056

Toll样受体4/核因子-κB信号通路 柴胡陷胸汤 脂多糖 人胃黏膜上皮GES-1细胞 机制

湖北省中医药管理局湖北省中医药重点学科建设项目国家中医药管理局国医大师传承工作室建设项目湖北中医药大学青苗计划国家自然科学基金青年科学基金项目

鄂中医通[2023]2号国中医药办人教函[2018]119号2020ZZX03081904076

2024

中华中医药杂志
中华中医药学会

中华中医药杂志

CSTPCD北大核心
影响因子：1.135
ISSN：1673-1727
年,卷(期)：2024.39(10)