Discussion on therapeutic effect of Taohe Chengqi Decoction on sepsis-associated encephalopathy based on the gut-choroid plexus vascular barrier
Objective:To investigate the therapeutic effect and mechanism of Taohe Chengqi Decoction on sepsis-associated encephalopathy(SAE).Methods:Seventy male SD rats were randomly divided into sham group,model group,0.5 times,1 times,2 times Taohe Chengqi Decoction group.Cecal ligation and puncture(CLP)method was used to construct the model.Open field experiment was used to observe the movement and exploration ability.The contents of TNF-α,IL-1β,IL-6 and LPS in serum and hippocampal tissue were determined by ELISA.The formation of Nissl bodies in hippocampus was observed by Nissl staining.The expression of PV-1 in intestinal and choroid plexus tissues were detected by immunofluorescence method.The expressions of PV-1,HIF-1α and VEGF-A in intestinal and choroid plexus tissues were detected by Western Blot.Results:Compared with the sham operation group,intraperitoneal infection,and the locomotor ability of rats was decreased obviously,inflammatory factors TNF-α,IL-1β,IL-6,LPS release in serum and hippocampal tissue were increased in model group(P＜0.01,P＜0.05),the expression of PV-1,HIF-1α and VEGF-A in the intestine and choroid plexus tissues increased(P＜0.01).Compared with model group,different concentrations of Taohe Chengqi Decoction could alleviate the abdominal infection,increase the locomotor and exploration ability,improve hippocampal neuron damage,and reduce the contents of TNF-α,IL-1β,IL-6 and LPS in serum and hippocampal tissue,among which the 1 times effect was the best.In addition,Taohe Chengqi Decoction could reduce the expression of PV-1,HIF-1α and VEGF-A in the intestine and choroid plexus tissues(P＜0.05,P＜0.01).Conclusion:Taohe Chengqi Decoction could treat SAE by improving the function of the gut-choroid plexus vascular barrier,and HIF-1α and VEGF-A may be the key regulatory molecules.
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