Discussion on the relieving pain mechanism of wheat grain moxibustion on sciatic nerve injury rats based on astrocyte activation and its mediated P38MAPK/CREB signaling pathway
Objective:To observe the effects of wheat grain moxibustion on astrocyte(AS)activation,gap junction protein 43(CX43),inflammatory factors and inflammatory signalling pathways in the lumbar(L)4-5 spinal cord of rats with sciatic nerve injury(SNI),and to explore the possible mechanism of wheat grain moxibustion in the treatment of sciatica.Methods:Male SD rats were randomly divided into blank group,sham-operated group,model group,and wheatgrain moxibustion group,with 6 rats in each group.The model group and the wheat grain moxibustion group used the classical clamp method to prepare the rat sciatic nerve severe compression injury model;the sham-operated group only exposed the sciatic nerve,not clamp treatment;the wheat grain moxibustion group took the affected side of the Huantiao(GB 30)to carry out the wheat grain moxibustion treatment in the 7th day after the modelling,and moxibustion was performed 6 times each time,so as to ensure that rats did not appear to struggle all over the body;the rest of the groups were fed normally,and were only fixed in the grasp,and did not do any other treatments;the intervention was carried out continuously for 10 d,1 time/d.The duration of cold stimulation and foot reduction in each group of rats on the 7th day after modelling and at the end of the intervention was observed,the pain threshold of rats in each group measured by fibre-wire nociceptor;the expression levels of interleukin-1β(IL-1β),interleukin-6(IL-6),tumour necrosis factor-α(TNF-α)and interferon-γ(IFN-γ)in the spinal cord tissue of rats were detected by ELISA,and the expression levels of interferon-γ(IFN-γ)in the spinal cord tissue of rats were detected by Western Blot.The expression levels of p38 mitogen-activated protease(P38MAPK),P-P38,glial fibrillary acidic protein(GFAP),cyclophosphoadenosine effector binding protein(CREB),P-CREB,and CX43 proteins were detected by Western Blot method in rat spinal cord tissues.Results:Before the intervention after modelling,compared with the sham-operated group,the mechanical pain thresholds of the rats in the model group and the wheat grain moxibustion group decreased(P＜0.01),and the time of foot reduction by cold stimulation was elevated(P＜0.01).After the intervention,compared with the model group,the rats in the wheat grain moxibustion group showed a significant increase in the thresholds of mechanical pain(P＜0.01),and the time of foot reduction by cold stimulation was shortened(P＜0.01).The expressiones of IL-1β,IL-6,TNF-α,IFN-γ in the spinal cord tissues and the protein expressiones of CX43,GFAP,P-P38 and P-CREB in the spinal cord tissues were decreased(P＜0.01,P＜0.05).Conclusion:The mechanism of treating Huantiao(GB 30)by wheat grain moxibustion can improve the pain response in SNI rats,which may be through the reduction of CX43,IFN-γ secretion,inhibition of AS activation,reduction of pro-inflammatory factor release,inhibition of inflammatory signalling pathway activation,and play an analgesic role.
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