人参皂苷Rg3对胰腺癌细胞血管生成拟态的抑制作用
Inhibitory effect of ginsenoside Rg3 on vasculogenic mimicry in pancreatic cancer
赵婷婷 1王兆洪 2林胜璋1
作者信息
- 1. 浙大城市学院医学院,杭州 310015
- 2. 温州医科大学附属第二医院,温州 325027
- 折叠
摘要
目的:探讨人参皂苷Rg3(Rg3)抑制胰腺癌细胞血管生成拟态(VM)的可能机制.方法:采用不同浓度的Rg3分别处理人胰腺癌SW1990细胞24h,MTT法检测细胞活力并计算IC50值;33 µmol/LRg3及25 nmol/LmiR-204抑制剂或阴性对照转染SW1990细胞24 h,MTT法检测细胞增殖;流式细胞术检测细胞凋亡;Transwell实验观察细胞迁移和侵袭情况;3D培养观察细胞VM的形成;RT-qPCR检测VE-cadherin和miR-204的表达水平;Western Blot检测血管内皮钙黏蛋白(VE-cadherin)的蛋白表达水平.结果:Rg3呈浓度依赖性抑制SW1990细胞增殖能力(P＜0.01),促进细胞凋亡,显著抑制细胞迁移(P＜0.01)、侵袭能力(P＜0.01)和VM形成(P＜0.01),并下调VE-cadherin mRNA和蛋白表达水平(P＜0.01).下调miR-204部分逆转了Rg3对SW1990细胞活力、迁移、侵袭和VM形成的抑制作用(P＜0.05),以及对细胞凋亡的促进作用.结论:Rg3通过上调miR-204抑制SW1990细胞增殖、迁移、侵袭能力和VM形成,促进凋亡.
Abstract
Objective:To explore the possible mechanism of ginsenoside Rg3(Rg3)inhibiting vasculogenic mimicry(VM)in pancreatic cancer.Methods:SW1990 cells were treated with different concentrations of Rg3 for 24 h.Cell viability was detected by MTT assay and IC50 value was calculated;SW1990 cells were transfected with 33 μmol/L Rg3 and 25 nmol/L miR-204 inhibitor or negative control for 24 h.Cell proliferation was detected by MTT;apoptosis was detected by flow cytometry;cell migration and invasion were observed by Transwell assay;3D culture to observe the formation of cellular VM;RT-qPCR to detect the expression level of VE-cadherin and miR-204;Western Blot to detect the protein expression level of VE-cadherin.Results:Rg3 inhibited SW1990 cell proliferation ability(P＜0.01)in a concentration-dependent manner,promoted apoptosis,significantly inhibited cell migration(P＜0.01),invasion ability(P＜0.01)and VM formation(P＜0.01),and down-regulated VE-cadherin mRNA and protein expression levels(P＜0.01).Down-regulation of miR-204 partially reversed the inhibitory effects of Rg3 on SW1990 cell viability,migration,invasion and VM formation(P＜0.05),and the promotion of apoptosis.Conclusion:Rg3 inhibited SW1990 cell proliferation,migration,invasion ability and VM formation,and promoted apoptosis through up-regulation of miR-204.
关键词
人参皂苷Rg3/胰腺癌/miR-204/血管生成拟态/迁移侵袭Key words
Ginsenoside Rg3/Pancreatic cancer/MiR-204/Vasculogenic mimicry/Migration and invasion引用本文复制引用
基金项目
浙江省自然科学基金重点项目(LZ24H280004)
出版年
2024
NETL
NSTL
万方数据