Inhibitory effect of ginsenoside Rg3 on vasculogenic mimicry in pancreatic cancer
Objective:To explore the possible mechanism of ginsenoside Rg3(Rg3)inhibiting vasculogenic mimicry(VM)in pancreatic cancer.Methods:SW1990 cells were treated with different concentrations of Rg3 for 24 h.Cell viability was detected by MTT assay and IC50 value was calculated;SW1990 cells were transfected with 33 μmol/L Rg3 and 25 nmol/L miR-204 inhibitor or negative control for 24 h.Cell proliferation was detected by MTT;apoptosis was detected by flow cytometry;cell migration and invasion were observed by Transwell assay;3D culture to observe the formation of cellular VM;RT-qPCR to detect the expression level of VE-cadherin and miR-204;Western Blot to detect the protein expression level of VE-cadherin.Results:Rg3 inhibited SW1990 cell proliferation ability(P＜0.01)in a concentration-dependent manner,promoted apoptosis,significantly inhibited cell migration(P＜0.01),invasion ability(P＜0.01)and VM formation(P＜0.01),and down-regulated VE-cadherin mRNA and protein expression levels(P＜0.01).Down-regulation of miR-204 partially reversed the inhibitory effects of Rg3 on SW1990 cell viability,migration,invasion and VM formation(P＜0.05),and the promotion of apoptosis.Conclusion:Rg3 inhibited SW1990 cell proliferation,migration,invasion ability and VM formation,and promoted apoptosis through up-regulation of miR-204.
Ginsenoside Rg3Pancreatic cancerMiR-204Vasculogenic mimicryMigration and invasion
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