Mechanism of Polygonati Rhizoma-Atractylodes Lancea regulating the progression of lung cancer through Apex1/STAT3 axis
Objective:To investigate the effects of Polygonati Rhizoma-Atractylodes Lancea on the development of non-small cell lung cancer(NSCLC)and its molecular mechanism.Methods:Human non-small cell lung cancer NCI-H1299 and PC9 cells were treated with Polygonati Rhizoma-Atractylodes Lancea.CCK-8 assay was used to evaluate the cell viability and Edu fluorescence staining was used to detect cell proliferation.C57BL/6 mice(KrasG12D/+;p53fl/fl)were used to establish a mouse model of primary lung cancer,and HE staining and immunohistochemistry were used to detect the progression of lung cancer.qPCR and Western Blot were used to analyze the expression level of Apex1 mRNA and protein,Western Blot and flow cytometry were used to analyze the expression level of STAT3,CDK1,p21,p27 and cell cycle.Results:After treated with Polygonati Rhizoma-Atractylodes Lancea for 48 hours,the proliferation of human non-small cell lung cancer NCI-H1299 and PC9 cells significantly decreased.The mouse model of primary lung cancer was successfully established,and compared with the control group,the progress of primary lung cancer in the treatment group was significantly inhibited.In the cell experiment in vitro,Polygonati Rhizoma-Atractylodes Lancea inhibited the expression of Apex1 and p-STAT3(P＜0.01,P＜0.05)in a concentration-dependent manner,down-regulated the expression of CDK1 protein(P＜0.01),increased the expression of p21 and p27 protein(P＜0.01),and blocked the cell cycle of NCI-H1299 and PC9 in a concentration-dependent manner,which was also verified in animal experiments.Conclusion:Polygonati Rhizoma-Atractylodes Lancea can inhibit the proliferation of NSCLC cells,slow down the progression of primary lung cancer in mice,and block the cell cycle of lung cancer cells by inhibiting Apex1/STAT3 signal pathway.
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