Effects of Paeoniae Radix Alba from different origins on the processing of vinegar-processed Paeoniae Radix Alba
Objective:To explore the differences of vinegar products processed by Paeoniae Radix Alba from different origins.Methods:Vinegar-processed Paeoniae Radix Alba were processed by Paeoniae Radix Alba from Anhui and Zhejiang.Chromatic meter was used to measure the chromatic values(L*,a*,b*)of vinegar-processed Paeoniae Radix Alba.Hot-dip method was used to measure the contents of water-soluble extract of vinegar-processed Paeoniae Radix Alba.High performance liquid chromatography(HPLC)was used to establish the characteristic chromatogram of vinegar-processed Paeoniae Radix Alba and evaluate the similarity.SIMCA 14.1 was used for principal components analysis(PCA)and discriminant analysis of orthogonal partial least squares(OPLS-DA).Results:Compared with the vinegar-processed Paeoniae Radix Alba processed by Hangbaishao,the vinegar-processed Paeoniae Radix Alba processed by Bobaishao had lighter color,higher L*,lower a*and b*,and higher water-soluble extract content.There were differences among the water-soluble extract of vinegar-processed Paeoniae Radix Alba processed by different batches of Bobaishao.A total of 6 common peaks were matched in 10 batches of HPLC characteristic chromatogram.The similarity of characteristic chromatogram ranged from 0.838 to 0.999.Six common peaks were identified,namely gallic acid,catechin,albiflorin,paeoniflorin,1,2,3,4,6-O-pentagalloylglucose,and benzoylpaeoniflorin.PCA and OPLS-DA results showed that 10 batches of vinegar-processed Paeoniae Radix Alba were clustered into two categories.a*,L*,peak-to-mass ratio of peak 4(paeoniflorin),b*,peak-to-mass ratio of peak 2(catechin)were selected as the index components that caused the quality difference of different batches of vinegar-processed Paeoniae Radix Alba.Conclusion:The appearance and internal quality of vinegar-processed Paeoniae Radix Alba can be effected by the origin of Paeoniae Radix Alba.It is necessary to fix the origin of the decoction pieces in the process of development and utilization.
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