Mechanism of acupuncture intervention on gut microbiota to enhance learning memory ability in APP/PS1 mice
Objective:To study the intervention of acupuncture on the learning memory ability of APP/PS1 mice by influencing the gut microbiota and its derivatives.Methods:Twenty-four double-transgenic APP/PS 1 mice aged 6 to 8 months were randomly allocated to the model group and the acupuncture group,with 12 mice in each group,along with twelve C57BL/6 mice serving as the control group.The acupuncture group received a single 30 minute session of acupuncture at the Danzhong(RN17),Zhongcun(RN12),and Zusanli(ST36)points,6 times a week for a total of 4 weeks of treatment.Mice Aβ levels were determined using Western Blot,lipopolysaccharide(LPS)levels were determined by ELISA,blood-brain barrier permeability was observed by immunofluorescence staining,and gut microbial structure was observed by 16S rDNA gene sequencing.Results:Compared with the control group,the model group exhibited longer evasion latency and swimming distance,fewer quadrant passes,the relative expression level of A β significantly increased(P＜0.01),the relative expression level of IL-6 and IL-1 β in both brain and serum significantly increased(P＜0.01),while the relative expression level of IL-10 and IL-4 in both brain and serum significantly decreased(P＜0.01),and the relative expression level of LPS in brain,serum,and colon significantly increased(P＜0.01).Compared with the model group,the acupuncture group exhibited lower evasion latency and swimming distance,more quadrant passes,the relative expression level of IL-6 and IL-1β in both brain and serum significantly decreased(P＜0.01),while the relative expression level of IL-10 and IL-4 in both brain and serum significantly increased(P＜0.01),and the relative expression level of LPS in brain,serum,and colon significantly decreased(P＜0.01),and the structural function of the gut microbiota could change.Conclusion:Acupuncture reduces Aβ deposition in the hippocampus of APP/PS1 mice and improves learning and memory by modulating the structural function of the gut microbiota.
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