Exploration on the mechanism of Xiaoyaosan in regulating glucose metabolism abnormalities in chronic stress rats based on the short chain fatty acid receptor pathway
Objective:To investigate the mechanism of glucose metabolism in rats in a chronic stress model based on the short-chain fatty acid receptor pathway by the Chinese herbal compound Xiaoyaosan.Methods:SD rats were randomly divided into normal,model,Fluoxetine and Xiaoyaosan groups,12 rats in each group,and fed for 1 week.The changes in blood glucose and behaviour of rats in each group were observed;ELISA was used to detect the contents of glucagon-like peptide-1(GLP-1)and peptide YY(PYY)in serum and colon tissue,and qRT-PCR was used to detect the expression changes of G protein-coupled receptors(GPR)41,GPR43,GLP-1,and PYY mRNA in rat colon tissue.Western Blot was used to detect the expression of GPR41 and GPR43 proteins in colon tissues.Results:Compared with the normal group,the number of central zone crossing and open entries in the model group rats was significantly reduced(P＜0.01),fasting blood glucose increased significantly from 2 to 6 weeks(P＜0.01),glucose tolerance test was significantly increased at 0,30 and 90 min(P＜0.01).GLP-1,PYY,GPR41,GPR43 mRNA expression decreased(P＜0.05,P＜0.01),the contents of GLP-1 and PYY in serum and colon tissues were significantly decreased(P＜0.05,P＜0.01)and the protein expressions of GPR41 and 43 were significantly decreased(P＜0.01).After the intervention of Xiaoyaosan,compared with the model group,the times of central region crossing and open-arm entry were significantly increased(P＜0.01),fasting blood glucose was significantly decreased at the 2nd,4th,5th and 6th weeks(P＜0.01,P＜0.05)and glucose tolerance was significantly decreased at 30 min.GLP-1,PYY,GPR41 and GPR43 mRNA expression significantly increased(P＜0.01,P＜0.05)in colon tissues,the contents of PYY and GLP-1 in colon tissues and serum were significantly increased(P＜0.01,P＜0.05),and the protein expressions of GPR41 and GPR43 in colon tissues were significantly increased(P＜0.01,P＜0.05).Conclusion:Xiaoyaosan ameliorates chronic stress-induced abnormalities in glucose metabolism in rats by modulating colonic GPR41,GPR43,GLP-1 and PYY expression changes.
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