Mechanism of alleviating inflammation reaction in rats with allergic rhinitis by regulating the Th1/Th2 balance with Shanlameiye Granules
Objective:To investigate the role and mechanism of Shanlameiye Granules in alleviating allergic rhinitis(AR).Methods:Sixty SD rats were randomly divided into normal control group,model control group,positive drug group(Rhinitis Spirit Tablet,0.432 g/kg),low,medium,and high dose groups of Shanlameiye Granules(Shanlameiye Granules,3.15,6.30,12.60 g/kg).Except for the normal control group,the model groups used ovalbumin(OVA)systemic and local attack methods to establish a rat model of AR.The nasal symptomatic behavior of rats was scored.Blood was taken after administration,blood cell analysis was performed and eosinophil count analysis in whole blood was performed by Giemsa staining.The contents of immunoglobulin E(IgE)and histamine(HIS)in serum and TNF-α,IL-10,IL-4 and IFN-α in rat nasal lavage solution were detected by ELISA.HE staining observed the histopathological changes of nasal mucosa in rats.Western Blot and RT-qPCR method detected STAT6,STAT4,GATA3,T-be,p65,IκB-α protein and mRNA expression in the nasal mucosa.Results:Compared with the blank control group,the behavioral scores of sneezing and nose scratching in the model group were significantly increased(P＜0.01),the number of leukocytes in the blood significantly increased(P＜0.01),the number of platelet in the blood significantly decreased(P＜0.01),and the number of eosinophils in the blood increased,and the levels of IgE and HIS in serum and TNF-α,IL-10 and IL-4 in nasal lavage fluid were significantly increased(P＜0.01,P＜0.05),the expression of STAT6,STAT4,GATA3,T-be,p65,and IκB-α in nasal mucosal tissues were significantly different(P＜0.01);nasal mucosal intertissue edema was pronounced.Compared with the model group,the above indexes were significantly improved in each dose group of Shanlameiye Granules,and the behavioral scores,the number of leukocytes significantly decreased(P＜0.01),and the number of eosinophils decreased,serum IgE and HIS levels significantly decreased(P＜0.01),TNF-α,IL-10 and IL-4 contents in nasal lavage solution in high dose group of shanlameiye Granules were significantly reduced(P＜0.05,P＜0.01),the content IFN-γ significantly increased(P＜0.01),the expression of STAT6,GATA3,T-bet and p65 in nasal mucosal tissues were significantly different(P＜0.05),and nasal mucosal hyperemia,edema and inflammatory cell infiltration in rats in the administration group were reduced.Conclusion:Shanlameiye Granules have a significant effect on the inflammation of AR rats,and the mechanism may be related to the regulation of Th1/Th2 immune balance.
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