Objective:To explore the protective effect of Buyang Huanwu Decoction on myocardial tissue and the influence of inflammatory and apoptotic factors in mice with diabetes cardiomyopathy(DCM).Methods:A total of 30 db/db mice(7-8 weeks old)were raised for 10 weeks to develop DCM and randomly divided into model group,Buyang Huanwu Decoction group,and Shuluodite group,with 10 mice in each group.Another 10 db/m mice(7-8 weeks old)were included in the control group.Corresponding medication treatment were provided for 12 weeks,the levels of blood glucose(FBG),creatine kinase isoenzyme(CK-MB),interleukin-6(IL-6),monocyte chemokine 1(MCP-1),tumor necrosis factor-α(TNF-α),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol(LDL-C)were detected.The mRNA expression of Toll like receptor(TLR4)and nuclear transcription factor-κB p65(NF-κB p65)were detected by qRT-PCR.Western Blot was used to detect the expression of TLR4,NF-κB p65,Cleaved caspase-3 and Cleaved PARP in mouse cardiac tissue.Immunohistochemical was used to detect the expression of TLR4,NF-κB p65,Ⅰ and Ⅲ collagen in cardiac tissue.HE,Masson,and Sirius red staining were used to observe the pathological and morphological changes of the heart.Results:Compared with the control group,the model group mice showed a significant decrease in the number of myocardial fibers,disordered arrangement of myocardial tissue and a significant increase in collagen fibers,the contents of FBG,CK-MB,IL-6,MCP-1,TNF-α,TG,LDL-C were significantly increased(P＜0.01,P＜0.05),while the HDL-C levels was decreased(P＜0.01),TNF-α,IL-6,and MCP-1 were significantly elevated(P＜0.01),at the same time,the expression of TLR4,NF-κB p65,Cleaved caspase-3,Cleaved PARP,and Ⅰ and Ⅲ collagen proteins was significantly enhanced in cardiac tissue(P＜0.01,P＜0.05).Compared with the model group,after intervention with Buyang Huanwu Decoction,the degree of cardiac pathological damage was significantly improved in DCM mice,the levels of CK-MB,IL-6,MCP-1,TNF-α,TG,and LDL-C significantly decreased(P＜0.01,P＜0.05),while the levels of HDL-C were significantly upregulated(P＜0.01),TNF-α,IL-6,and MCP-1 were decreased(P＜0.01,P＜0.05),the expression of TLR4,NF-κB p65,Cleaved caspase-3,Cleaved PARP,Ⅰ and Ⅲ collagen was significantly reduced(P＜0.01,P＜0.05).Conclusion:Buyang Huanwu Decoction can exert anti-inflammatory and anti-apoptotic effects on DCM mice by inhibiting the TLR4/NF-κB pathway and pro-apoptotic factor expression,reducing cardiac pathological damage,and thus exerting cardioprotective effects.
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