Objective:To investigate the effects of Zigui Yichong Formula on the expression of SLC7A11 and GPX4 mNRA related to ovarian ferroptosis in rats with diminished ovarian reserve(DOR).Methods:Forty healthy female SD rats were randomly divided into normal group,model group,traditional Chinese medicine(TCM)group and coenzyme Q10 group according to body weight,10 rats in each group.In addition to the normal group,DOR models rat were prepared by intraperitoneal injection of cyclophosphamide,and the corresponding treatment factors were given to each group for 14 days,respectively.The rats were killed in the pre-estrus period.Serum levels of estradiol(E2),anti-Müllerian hormone(AMH),follicle stimulating hormone(FSH)and luteinizing hormone(LH)were determined by ELISA.The hisopathological changes of ovarian tissue were observed by HE staining.The expression of reactive oxygen species(ROS)in rat ovarian tissue was detected by immunofluorescence chemistry.mRNA expression of SLC7A11 and GPX4 in rat ovarian tissue was detected by RT-PCR.Results:Compared with normal group,the levels of serum E2 and AMH in model group significantly decreased(P＜0.05),while the levels of FSH and LH significantly increased(P＜0.05),the number of granulosa cell layers was reduced and the arrangement was loose,ROS was highly expressed in ovarian tissue,the expressions of SLC7A11 and GPX4 mRNA in ovarian tissue were significantly down-regulated(P＜0.05).Compared with model group,the levels of serum E2 and AMH in TCM group and coenzyme Q10 group significantly increased(P＜0.05),while the levels of FSH and LH significantly decreased(except for LH in TCM group)(P＜0.05),the number of follicles and corpus luteum at all levels of ovary increased slightly,the number of follicular granulosa cells increased,the expression of ROS in ovarian tissue was decreased,the mRNA expression levels of SLC7A11 and GPX4 in ovarian tissue were increased(P＜0.05).Conclusion:The mechanism of Zigui Yichong Formula in the prevention and treatment of DOR may be related to reducing oxidative stress in ovarian tissue and improving the expression level of genes related to ferroptosis in DOR model rats.
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