Optimization of phenol-sulfuric acid determination conditions of polysaccharide in Anoectochilu roxburghii(wall) Lindl by orthogonal test
OBJECTIVE To establish quantitative analysis method for polysaccharide from Anoectochilus roxburghii (wall) Lindl. METHODS Polysaccharide was obtained with the ultrasound extraction and alcohol precipitation method, phenol-sulfuric acid method was used for content deter mination glucose as parameters. The absorbance is measured at 490 nm. L_9 (3~4) orthogo-nal design was chosen for optimum measuing conditions. RESULTS When the sample solution used 1.0 mL,0.6 mL of 5% so-lution of phenol,sulfuric acid 6.0 mL, 100 ℃ water bath for 15 min reaction conditions determination, the results is best. The content of crude polysaccharide was 12.09% in A. roxburghii. CONCLUSION The method after optimization was rapid with good reproducibility, simple and accurate.
A. roxburghiipolysaccharidehenol-sulfuric acid method
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