Study on the fingerprint of different kinds of dandelion by HPCE
OBJECTIVE To research and construct the HPCE characteristic fingerprint of dandelion;to provide a new method for quality of dandelion. METHODS The separation on capillary zone electrophoresis (CZE) was used to determine the fingerprints. The buffer was 20 mmol· L-1 borate solution(pH = 9. 18);the applied voltage was 15 kV and the detection wavelength was 244 nm. The caffeic acid was used as a reference standard. The results were analyzed by Hierarchical Cluster and different Similarity Evaluation Systems. RESULTS Fingerprint was established with 13 common peaks of 10 samples with similarity of 0. 926-0. 983. CONCLUSION The method is simple, accurate, good reproducibility, and suitable to the quality control of dandelion.